Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21138 | 5' | -55.7 | NC_004775.1 | + | 17980 | 0.66 | 0.580326 |
Target: 5'- aCACGUGACggcaUGCGCUuGUCuGCAaauACCa -3' miRNA: 3'- -GUGUACUG----GCGCGGuCAGuCGU---UGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 34104 | 0.66 | 0.580326 |
Target: 5'- gAUAUGACCGCcgGCgaucaGGUUAcGCAGCCa -3' miRNA: 3'- gUGUACUGGCG--CGg----UCAGU-CGUUGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 26075 | 0.66 | 0.576955 |
Target: 5'- uGCGUGACgCGCaCCAGgucgccgguguacuUCAuGCAGCCUu -3' miRNA: 3'- gUGUACUG-GCGcGGUC--------------AGU-CGUUGGA- -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 32876 | 0.66 | 0.557942 |
Target: 5'- --uGUGGCCGgGCCAauGcCAGCAGCa- -3' miRNA: 3'- gugUACUGGCgCGGU--CaGUCGUUGga -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 31574 | 0.66 | 0.557942 |
Target: 5'- uGCAUGGCCuGCGCCGuGUaauGC-ACCg -3' miRNA: 3'- gUGUACUGG-CGCGGU-CAgu-CGuUGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 10635 | 0.67 | 0.513991 |
Target: 5'- gCAgAUGGCC-CGCCAGacuuUCAGCGGgCUu -3' miRNA: 3'- -GUgUACUGGcGCGGUC----AGUCGUUgGA- -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 5109 | 0.67 | 0.513991 |
Target: 5'- aCACAUGACCuuuCuCCAG-CAGUGACCa -3' miRNA: 3'- -GUGUACUGGc--GcGGUCaGUCGUUGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 18676 | 0.67 | 0.492545 |
Target: 5'- -uCGUGACUGCGCUGGUCAuCucACCa -3' miRNA: 3'- guGUACUGGCGCGGUCAGUcGu-UGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 14974 | 0.68 | 0.47152 |
Target: 5'- aUACGUGaaGCCGgaUGCCGGUagccagggcaCAGCAGCCg -3' miRNA: 3'- -GUGUAC--UGGC--GCGGUCA----------GUCGUUGGa -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 38685 | 0.7 | 0.356217 |
Target: 5'- uUACAaGGCUGCGCUGG-CAGCAGCa- -3' miRNA: 3'- -GUGUaCUGGCGCGGUCaGUCGUUGga -5' |
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21138 | 5' | -55.7 | NC_004775.1 | + | 38718 | 1.07 | 0.000741 |
Target: 5'- cCACAUGACCGCGCCAGUCAGCAACCUc -3' miRNA: 3'- -GUGUACUGGCGCGGUCAGUCGUUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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