Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21140 | 5' | -49.2 | NC_004775.1 | + | 19556 | 0.66 | 0.913713 |
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Target: 5'- cGuAGCuguuCGGCACAGuaaucgagauGCGUUUGCAGa -3' miRNA: 3'- aC-UCGu---GUUGUGUCuu--------CGUAAGCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 741 | 0.66 | 0.913713 |
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Target: 5'- aGAGgAUGGCACAGA-GCuggCGCAu -3' miRNA: 3'- aCUCgUGUUGUGUCUuCGuaaGCGUc -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 17020 | 0.66 | 0.906689 |
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Target: 5'- cGAGCAaagcaaGGCACuugGGGAGCAgcguuucCGCAGc -3' miRNA: 3'- aCUCGUg-----UUGUG---UCUUCGUaa-----GCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 3132 | 0.66 | 0.891686 |
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Target: 5'- aGAGCGCcGCcgccguGCAGcAGCAggcCGCAGu -3' miRNA: 3'- aCUCGUGuUG------UGUCuUCGUaa-GCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 33218 | 0.67 | 0.866002 |
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Target: 5'- --uGCGCuuugccgAACGgGuGAAGCGUUCGCAGa -3' miRNA: 3'- acuCGUG-------UUGUgU-CUUCGUAAGCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 4875 | 0.67 | 0.857117 |
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Target: 5'- cGAGCGCAuuaaccGCuaucgcuaaugcaGCAGGAGCAc-CGCAGc -3' miRNA: 3'- aCUCGUGU------UG-------------UGUCUUCGUaaGCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 18521 | 0.67 | 0.848884 |
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Target: 5'- -aAGCAUuuGACGCuGAAGCAga-GCAGa -3' miRNA: 3'- acUCGUG--UUGUGuCUUCGUaagCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 14034 | 0.68 | 0.788867 |
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Target: 5'- -uGGCGCaAugGCAGAGGCuaccugUCGCAu -3' miRNA: 3'- acUCGUG-UugUGUCUUCGua----AGCGUc -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 39254 | 0.72 | 0.591657 |
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Target: 5'- aGAGCGCAAaGCAGcgcAGCGUgCGCGGc -3' miRNA: 3'- aCUCGUGUUgUGUCu--UCGUAaGCGUC- -5' |
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| 21140 | 5' | -49.2 | NC_004775.1 | + | 1117 | 1.11 | 0.001815 |
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Target: 5'- cUGAGCACAACACAGAAGCAUUCGCAGg -3' miRNA: 3'- -ACUCGUGUUGUGUCUUCGUAAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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