Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21141 | 3' | -52.6 | NC_004775.1 | + | 27242 | 0.71 | 0.488244 |
Target: 5'- uUCUgGCUGACaguacccGGGCAAUGAaacaggccguuaucaAGCGCGa -3' miRNA: 3'- -GGAgUGACUGc------UCCGUUACU---------------UCGCGC- -5' |
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21141 | 3' | -52.6 | NC_004775.1 | + | 20885 | 0.72 | 0.422515 |
Target: 5'- cCCUCGCaGugGGuaauGGCGcuuUGAAGCGCa -3' miRNA: 3'- -GGAGUGaCugCU----CCGUu--ACUUCGCGc -5' |
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21141 | 3' | -52.6 | NC_004775.1 | + | 34354 | 0.78 | 0.199842 |
Target: 5'- gCUCGCUGAUGAGGUGGaaGAAGUGUGg -3' miRNA: 3'- gGAGUGACUGCUCCGUUa-CUUCGCGC- -5' |
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21141 | 3' | -52.6 | NC_004775.1 | + | 1489 | 1.11 | 0.000999 |
Target: 5'- gCCUCACUGACGAGGCAAUGAAGCGCGu -3' miRNA: 3'- -GGAGUGACUGCUCCGUUACUUCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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