Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21142 | 5' | -53.6 | NC_004775.1 | + | 9464 | 0.66 | 0.628292 |
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Target: 5'- aUCAcagcaguGGCUGGCgAugGCCUGACagcaaCUGc -3' miRNA: 3'- aAGU-------UUGACCG-UugCGGACUGg----GACu -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 18470 | 0.68 | 0.560139 |
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Target: 5'- ----uACUGGCAGCGCUUGGagaucuaccgCCUGAg -3' miRNA: 3'- aaguuUGACCGUUGCGGACUg---------GGACU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 753 | 0.68 | 0.560139 |
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Target: 5'- --aGAGCUGGCGcAUGCCaccgGGCCCaGAa -3' miRNA: 3'- aagUUUGACCGU-UGCGGa---CUGGGaCU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 15147 | 0.69 | 0.504034 |
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Target: 5'- gUCGAACUGaaagcgguaGCAGCGCCgacugugGGCCCgccGAa -3' miRNA: 3'- aAGUUUGAC---------CGUUGCGGa------CUGGGa--CU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 25081 | 0.69 | 0.471555 |
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Target: 5'- aUCAGGCUGGCuACGCCgauaaggauUGAUgCUGu -3' miRNA: 3'- aAGUUUGACCGuUGCGG---------ACUGgGACu -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 4661 | 0.69 | 0.471555 |
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Target: 5'- -aCAGGCUGGUGAgGCCgauagauuucUGAgCCUGAg -3' miRNA: 3'- aaGUUUGACCGUUgCGG----------ACUgGGACU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 8948 | 0.69 | 0.460965 |
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Target: 5'- --uGAGCUGGCGAUGCCguaugcUGACaCUGAc -3' miRNA: 3'- aagUUUGACCGUUGCGG------ACUGgGACU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 3531 | 0.71 | 0.371857 |
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Target: 5'- -cCGGGCUGGUGAUGCCUGACaucaugccgCUGGa -3' miRNA: 3'- aaGUUUGACCGUUGCGGACUGg--------GACU- -5' |
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| 21142 | 5' | -53.6 | NC_004775.1 | + | 3503 | 1.06 | 0.001146 |
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Target: 5'- gUUCAAACUGGCAACGCCUGACCCUGAc -3' miRNA: 3'- -AAGUUUGACCGUUGCGGACUGGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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