Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21143 | 3' | -51.3 | NC_004775.1 | + | 4168 | 1.09 | 0.001511 |
Target: 5'- gGUUGCCCCGACAUCGCUCAAGAAUCAg -3' miRNA: 3'- -CAACGGGGCUGUAGCGAGUUCUUAGU- -5' |
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21143 | 3' | -51.3 | NC_004775.1 | + | 11308 | 0.72 | 0.490236 |
Target: 5'- --gGCCCCGGCAUCGaugaUCGgcggaagcGGAAUCc -3' miRNA: 3'- caaCGGGGCUGUAGCg---AGU--------UCUUAGu -5' |
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21143 | 3' | -51.3 | NC_004775.1 | + | 12279 | 0.7 | 0.568983 |
Target: 5'- --cGUgCCGACGUUGCUCAGGc-UCAg -3' miRNA: 3'- caaCGgGGCUGUAGCGAGUUCuuAGU- -5' |
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21143 | 3' | -51.3 | NC_004775.1 | + | 36195 | 0.69 | 0.615435 |
Target: 5'- -cUGCCCUGGCAUugcaccgaccUGCUCAAGGuaugCAu -3' miRNA: 3'- caACGGGGCUGUA----------GCGAGUUCUua--GU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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