Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21143 | 5' | -52.3 | NC_004775.1 | + | 21346 | 0.66 | 0.810838 |
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Target: 5'- aUUGUUGgC-GGCGGUGaauggcuUGGCGUugCa -3' miRNA: 3'- gGACAACgGaCCGCUAC-------ACUGCAugG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 29866 | 0.66 | 0.80198 |
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Target: 5'- uCCUGUguccaUGCacaGCGuuuaucUGUGAUGUACCa -3' miRNA: 3'- -GGACA-----ACGgacCGCu-----ACACUGCAUGG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 7394 | 0.67 | 0.750199 |
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Target: 5'- cUCUGcgaGCCUGGCGAuauUGUGGaGUuuuCCg -3' miRNA: 3'- -GGACaa-CGGACCGCU---ACACUgCAu--GG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 15420 | 0.68 | 0.706386 |
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Target: 5'- aCCU--UGCC-GcGCGAaaUGcUGACGUGCCg -3' miRNA: 3'- -GGAcaACGGaC-CGCU--AC-ACUGCAUGG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 30210 | 0.68 | 0.672663 |
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Target: 5'- ----aUGCCaaGGCGAUGUuuaaGCGUGCCg -3' miRNA: 3'- ggacaACGGa-CCGCUACAc---UGCAUGG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 28796 | 0.71 | 0.537055 |
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Target: 5'- ----aUGCgaGGUGcgGUGACGUGCUg -3' miRNA: 3'- ggacaACGgaCCGCuaCACUGCAUGG- -5' |
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| 21143 | 5' | -52.3 | NC_004775.1 | + | 4203 | 1.14 | 0.000641 |
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Target: 5'- cCCUGUUGCCUGGCGAUGUGACGUACCu -3' miRNA: 3'- -GGACAACGGACCGCUACACUGCAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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