Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21144 | 3' | -52.9 | NC_004775.1 | + | 12457 | 0.66 | 0.700715 |
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Target: 5'- cGGAagGUGCCcgggcUGGUUUuauGCAGCAGg -3' miRNA: 3'- -UCUagUACGGuu---ACCAGGu--CGUCGUC- -5' |
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| 21144 | 3' | -52.9 | NC_004775.1 | + | 37269 | 0.66 | 0.689227 |
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Target: 5'- cGGAUCGUugUAGUGGUCUugcauGUAGCGGa -3' miRNA: 3'- -UCUAGUAcgGUUACCAGGu----CGUCGUC- -5' |
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| 21144 | 3' | -52.9 | NC_004775.1 | + | 758 | 0.67 | 0.642789 |
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Target: 5'- uGGcgCAUGCCAccgGGcCCAGaaaguGGCAGg -3' miRNA: 3'- -UCuaGUACGGUua-CCaGGUCg----UCGUC- -5' |
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| 21144 | 3' | -52.9 | NC_004775.1 | + | 5811 | 0.68 | 0.596169 |
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Target: 5'- uGGAUCA--CCGAauUGGUCCAGUgcacguugAGCAGc -3' miRNA: 3'- -UCUAGUacGGUU--ACCAGGUCG--------UCGUC- -5' |
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| 21144 | 3' | -52.9 | NC_004775.1 | + | 7360 | 0.69 | 0.527449 |
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Target: 5'- cGGUUAcuuUGCCAGUGGUCgGcGCGGUAc -3' miRNA: 3'- uCUAGU---ACGGUUACCAGgU-CGUCGUc -5' |
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| 21144 | 3' | -52.9 | NC_004775.1 | + | 5615 | 1.08 | 0.001021 |
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Target: 5'- aAGAUCAUGCCAAUGGUCCAGCAGCAGc -3' miRNA: 3'- -UCUAGUACGGUUACCAGGUCGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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