Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21144 | 5' | -53.8 | NC_004775.1 | + | 3507 | 0.65 | 0.709016 |
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Target: 5'- uGCCgcUGGaCAGAaugcgcuGAGCCAUUGAGcCAGUa -3' miRNA: 3'- -CGG--ACC-GUCU-------UUUGGUGGCUC-GUCAc -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 36146 | 0.66 | 0.67623 |
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Target: 5'- uGCCagGGCAGGGAA-CAgCGAGCAuGUu -3' miRNA: 3'- -CGGa-CCGUCUUUUgGUgGCUCGU-CAc -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 15833 | 0.66 | 0.675091 |
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Target: 5'- cGCCU-GCAGGAuGCCACCGAcacgcuuGCAc-- -3' miRNA: 3'- -CGGAcCGUCUUuUGGUGGCU-------CGUcac -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 26519 | 0.67 | 0.641913 |
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Target: 5'- -aCUGGCAGAGuacGCUgAUCGGGCAGc- -3' miRNA: 3'- cgGACCGUCUUu--UGG-UGGCUCGUCac -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 38123 | 0.67 | 0.618968 |
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Target: 5'- ---aGGCAGAAAAaauaGCCGAGUGGUa -3' miRNA: 3'- cggaCCGUCUUUUgg--UGGCUCGUCAc -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 756 | 0.68 | 0.573308 |
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Target: 5'- -gCUGGCGcau-GCCACCGGGCccagaaAGUGg -3' miRNA: 3'- cgGACCGUcuuuUGGUGGCUCG------UCAC- -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 17413 | 0.68 | 0.550754 |
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Target: 5'- uGCCUGGCGGGcAAACUcaAUCG-GCGGcUGg -3' miRNA: 3'- -CGGACCGUCU-UUUGG--UGGCuCGUC-AC- -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 34114 | 0.73 | 0.315118 |
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Target: 5'- cGCCUGGCGcGAuauGACCGCCG-GCGa-- -3' miRNA: 3'- -CGGACCGU-CUu--UUGGUGGCuCGUcac -5' |
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| 21144 | 5' | -53.8 | NC_004775.1 | + | 5650 | 1.12 | 0.000541 |
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Target: 5'- aGCCUGGCAGAAAACCACCGAGCAGUGg -3' miRNA: 3'- -CGGACCGUCUUUUGGUGGCUCGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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