Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21148 | 3' | -53.6 | NC_004775.1 | + | 27054 | 0.66 | 0.739719 |
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Target: 5'- aACGCUGGucauuggucaguauGGUUGUgacggUAUCAGUCUGGc -3' miRNA: 3'- -UGCGGCC--------------UUAGCGa----AUAGUCAGGCCu -5' |
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| 21148 | 3' | -53.6 | NC_004775.1 | + | 2352 | 0.66 | 0.68774 |
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Target: 5'- --aCCGGAGaaagCGCcUGUgcCAGUCCGGAa -3' miRNA: 3'- ugcGGCCUUa---GCGaAUA--GUCAGGCCU- -5' |
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| 21148 | 3' | -53.6 | NC_004775.1 | + | 36341 | 0.68 | 0.597007 |
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Target: 5'- cGCGCCGcGuccuuGUCGCUgccgAUaAGUCCGGc -3' miRNA: 3'- -UGCGGC-Cu----UAGCGAa---UAgUCAGGCCu -5' |
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| 21148 | 3' | -53.6 | NC_004775.1 | + | 15521 | 0.69 | 0.54097 |
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Target: 5'- cCGCCGGGAUCGgUgcuUCAGaaUCUGGc -3' miRNA: 3'- uGCGGCCUUAGCgAau-AGUC--AGGCCu -5' |
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| 21148 | 3' | -53.6 | NC_004775.1 | + | 16478 | 0.73 | 0.342632 |
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Target: 5'- gUGUCGGGAUgCGUaaUUGUCAGaUCCGGAu -3' miRNA: 3'- uGCGGCCUUA-GCG--AAUAGUC-AGGCCU- -5' |
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| 21148 | 3' | -53.6 | NC_004775.1 | + | 14311 | 1.09 | 0.00104 |
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Target: 5'- uACGCCGGAAUCGCUUAUCAGUCCGGAg -3' miRNA: 3'- -UGCGGCCUUAGCGAAUAGUCAGGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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