Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21148 | 5' | -47.9 | NC_004775.1 | + | 5262 | 0.66 | 0.953065 |
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Target: 5'- aGCAAccuGCUGAUGguGgugCAGCUCCu -3' miRNA: 3'- cUGUUuu-UGACUACguCg--GUUGAGGc -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 14699 | 0.66 | 0.937557 |
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Target: 5'- aGACGAuaAAACa---GCAGCCAAC-CCGg -3' miRNA: 3'- -CUGUU--UUUGacuaCGUCGGUUGaGGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 32223 | 0.67 | 0.919108 |
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Target: 5'- aGCu--GAUUGAUGCAGUCAGcCUCUc -3' miRNA: 3'- cUGuuuUUGACUACGUCGGUU-GAGGc -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 37049 | 0.67 | 0.905167 |
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Target: 5'- cGAUAAAucCUGcaucccAUGCGGCCAGCaUUCGg -3' miRNA: 3'- -CUGUUUuuGAC------UACGUCGGUUG-AGGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 3163 | 0.68 | 0.881022 |
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Target: 5'- cACGAGAACUGAcaacugcUGCAuCCuGCUCCu -3' miRNA: 3'- cUGUUUUUGACU-------ACGUcGGuUGAGGc -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 1701 | 0.68 | 0.864764 |
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Target: 5'- ----cAGGCUGAUGCAGUauuuauCGACUUCGg -3' miRNA: 3'- cuguuUUUGACUACGUCG------GUUGAGGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 31743 | 0.69 | 0.846526 |
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Target: 5'- aGCAGAGAUcGAUGCAGaggCGGCUuCCGg -3' miRNA: 3'- cUGUUUUUGaCUACGUCg--GUUGA-GGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 26521 | 0.72 | 0.694697 |
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Target: 5'- uGGCAGAGuacGCUGAUcggGCAGCCGAagCCGg -3' miRNA: 3'- -CUGUUUU---UGACUA---CGUCGGUUgaGGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 29292 | 0.73 | 0.610924 |
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Target: 5'- cGCAGAguugaauaaaccAGCgUGAUGCuGCCGACUCUGa -3' miRNA: 3'- cUGUUU------------UUG-ACUACGuCGGUUGAGGC- -5' |
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| 21148 | 5' | -47.9 | NC_004775.1 | + | 14348 | 1.1 | 0.002816 |
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Target: 5'- uGACAAAAACUGAUGCAGCCAACUCCGa -3' miRNA: 3'- -CUGUUUUUGACUACGUCGGUUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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