Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21151 | 3' | -53.8 | NC_004775.1 | + | 31890 | 0.68 | 0.483296 |
Target: 5'- uAAGCCAGuagccccuucuaAGCAGAaagUUUUCACGCGUg -3' miRNA: 3'- cUUCGGUC------------UCGUCUa--GAGAGUGCGUA- -5' |
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21151 | 3' | -53.8 | NC_004775.1 | + | 31758 | 0.7 | 0.410693 |
Target: 5'- cGAGGCCuguuucuucggAGAGCAGGUCaaUUACGUAUg -3' miRNA: 3'- -CUUCGG-----------UCUCGUCUAGagAGUGCGUA- -5' |
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21151 | 3' | -53.8 | NC_004775.1 | + | 8212 | 0.73 | 0.258266 |
Target: 5'- -cAGCCAGAGCAGcAUCgcgaCACGCAUc -3' miRNA: 3'- cuUCGGUCUCGUC-UAGaga-GUGCGUA- -5' |
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21151 | 3' | -53.8 | NC_004775.1 | + | 15889 | 1.05 | 0.001197 |
Target: 5'- cGAAGCCAGAGCAGAUCUCUCACGCAUu -3' miRNA: 3'- -CUUCGGUCUCGUCUAGAGAGUGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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