Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21152 | 5' | -56.3 | NC_004775.1 | + | 17333 | 1.06 | 0.000683 |
Target: 5'- uUAUCUGGUCGCCAGCCGCCGAUUGAGu -3' miRNA: 3'- -AUAGACCAGCGGUCGGCGGCUAACUC- -5' |
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21152 | 5' | -56.3 | NC_004775.1 | + | 25362 | 0.71 | 0.289498 |
Target: 5'- aAUCUGGUaucacaCGCCaauGGCCGCUGAUUuaucaGAGg -3' miRNA: 3'- aUAGACCA------GCGG---UCGGCGGCUAA-----CUC- -5' |
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21152 | 5' | -56.3 | NC_004775.1 | + | 26437 | 0.68 | 0.418553 |
Target: 5'- uUGUCggcgcugGGUCGUCAGCCGCaGAcaccgGAGu -3' miRNA: 3'- -AUAGa------CCAGCGGUCGGCGgCUaa---CUC- -5' |
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21152 | 5' | -56.3 | NC_004775.1 | + | 16593 | 0.66 | 0.543607 |
Target: 5'- --cUUGGcCGCCAGCCaaagGCUGAU-GAGc -3' miRNA: 3'- auaGACCaGCGGUCGG----CGGCUAaCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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