Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21153 | 3' | -50.9 | NC_004775.1 | + | 6866 | 0.66 | 0.870451 |
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Target: 5'- --aUAGCCUGuuUGugCA--GCCAUGCg -3' miRNA: 3'- cgcAUUGGACu-ACugGUcaCGGUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 26075 | 0.66 | 0.853162 |
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Target: 5'- uGCGUGacgcgcACCaGGUcGCCGGUGUacuuCAUGCa -3' miRNA: 3'- -CGCAU------UGGaCUAcUGGUCACG----GUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 27365 | 0.66 | 0.844125 |
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Target: 5'- uUGUcgAACgUGGUGGCCGGgcuacaGCgGUGCa -3' miRNA: 3'- cGCA--UUGgACUACUGGUCa-----CGgUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 13411 | 0.67 | 0.815563 |
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Target: 5'- cCGUGugCgcGAUG-CCAGUGCggcguaCAUGCg -3' miRNA: 3'- cGCAUugGa-CUACuGGUCACG------GUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 25589 | 0.67 | 0.795428 |
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Target: 5'- ---aAGCCaGAUGACCAG-GCgAUGUu -3' miRNA: 3'- cgcaUUGGaCUACUGGUCaCGgUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 6566 | 0.67 | 0.77453 |
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Target: 5'- -aGUGACggcaaUGgcGGCCAGUuCCGUGCg -3' miRNA: 3'- cgCAUUGg----ACuaCUGGUCAcGGUACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 14976 | 0.68 | 0.752975 |
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Target: 5'- aCGUGaaGCCgGAUG-CCGGUaGCCAggGCa -3' miRNA: 3'- cGCAU--UGGaCUACuGGUCA-CGGUa-CG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 5092 | 0.72 | 0.535446 |
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Target: 5'- aGCaGUGACCaGAUGACgCGGcgGCCcUGCu -3' miRNA: 3'- -CG-CAUUGGaCUACUG-GUCa-CGGuACG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 34037 | 0.77 | 0.271115 |
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Target: 5'- uGCGUAACCUGAUcGCCGGcgGUCAUaucGCg -3' miRNA: 3'- -CGCAUUGGACUAcUGGUCa-CGGUA---CG- -5' |
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| 21153 | 3' | -50.9 | NC_004775.1 | + | 17883 | 1.14 | 0.000776 |
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Target: 5'- uGCGUAACCUGAUGACCAGUGCCAUGCu -3' miRNA: 3'- -CGCAUUGGACUACUGGUCACGGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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