miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21155 3' -51.6 NC_004775.1 + 14430 0.66 0.828352
Target:  5'- -uGGCGGUgaugugGUAAUGCCAU--UUGGCa -3'
miRNA:   3'- guCCGUCA------UAUUGCGGUAacAGCCGc -5'
21155 3' -51.6 NC_004775.1 + 14338 0.66 0.816726
Target:  5'- aCAGGUuGUAgcuuagcuggcGCGCCuggggGUUGUUGGCGa -3'
miRNA:   3'- -GUCCGuCAUau---------UGCGG-----UAACAGCCGC- -5'
21155 3' -51.6 NC_004775.1 + 37179 0.67 0.777958
Target:  5'- uGGGCGGcgAUAccuucgagaACGCCA---UCGGCGa -3'
miRNA:   3'- gUCCGUCa-UAU---------UGCGGUaacAGCCGC- -5'
21155 3' -51.6 NC_004775.1 + 648 0.71 0.495058
Target:  5'- -cGGCGGgccuuCGCCAUucgGUCGGCa -3'
miRNA:   3'- guCCGUCauauuGCGGUAa--CAGCCGc -5'
21155 3' -51.6 NC_004775.1 + 26419 1.08 0.001746
Target:  5'- uCAGGCAGUAUAACGCCAUUGUCGGCGc -3'
miRNA:   3'- -GUCCGUCAUAUUGCGGUAACAGCCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.