miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21156 3' -53.2 NC_004775.1 + 38724 0.66 0.706427
Target:  5'- aACCgGCCACAUGaccgcGCCAGuCAG-CAAc- -3'
miRNA:   3'- -UGGgUGGUGUAU-----CGGUC-GUCuGUUag -5'
21156 3' -53.2 NC_004775.1 + 14479 0.66 0.695
Target:  5'- gACCguuaCACCGCAUcuGCgCAGCAGGCGc-- -3'
miRNA:   3'- -UGG----GUGGUGUAu-CG-GUCGUCUGUuag -5'
21156 3' -53.2 NC_004775.1 + 39560 0.66 0.671956
Target:  5'- -aCCAUCGCAcGGacacgacuuaaCCGGCAGugAAUCg -3'
miRNA:   3'- ugGGUGGUGUaUC-----------GGUCGUCugUUAG- -5'
21156 3' -53.2 NC_004775.1 + 34177 0.67 0.625462
Target:  5'- cGCCuCGCCGgAUGGCCAGaugauaaaACAGUCu -3'
miRNA:   3'- -UGG-GUGGUgUAUCGGUCguc-----UGUUAG- -5'
21156 3' -53.2 NC_004775.1 + 10641 0.67 0.601047
Target:  5'- gGCCCGCCAgacuuuCAgcgGGCUugcgcaucucgaaGGcCAGACAGUCa -3'
miRNA:   3'- -UGGGUGGU------GUa--UCGG-------------UC-GUCUGUUAG- -5'
21156 3' -53.2 NC_004775.1 + 17423 0.68 0.533447
Target:  5'- cAUCCACC-CAU-GCCuGGCGGGCAAacUCa -3'
miRNA:   3'- -UGGGUGGuGUAuCGG-UCGUCUGUU--AG- -5'
21156 3' -53.2 NC_004775.1 + 3232 0.7 0.436397
Target:  5'- cACCCugCGCAccAGUCAGCAGAguAg- -3'
miRNA:   3'- -UGGGugGUGUa-UCGGUCGUCUguUag -5'
21156 3' -53.2 NC_004775.1 + 2043 0.71 0.415228
Target:  5'- aGCaCCugCGCAUuccuggccaggagAGCCAGCAGgguaaaGCGGUCa -3'
miRNA:   3'- -UG-GGugGUGUA-------------UCGGUCGUC------UGUUAG- -5'
21156 3' -53.2 NC_004775.1 + 778 0.71 0.403417
Target:  5'- cCCCACggaaacgcguacagCGCAUAGCCAGCGGGuCAu-- -3'
miRNA:   3'- uGGGUG--------------GUGUAUCGGUCGUCU-GUuag -5'
21156 3' -53.2 NC_004775.1 + 29022 1.1 0.000677
Target:  5'- aACCCACCACAUAGCCAGCAGACAAUCa -3'
miRNA:   3'- -UGGGUGGUGUAUCGGUCGUCUGUUAG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.