Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21157 | 5' | -53.1 | NC_004775.1 | + | 29344 | 1.06 | 0.001384 |
Target: 5'- gUUAGUCAUACACCACCCCGCGACAUCc -3' miRNA: 3'- -AAUCAGUAUGUGGUGGGGCGCUGUAG- -5' |
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21157 | 5' | -53.1 | NC_004775.1 | + | 1034 | 0.68 | 0.566156 |
Target: 5'- gUGGuUCAccUGCACCGCUaCCGCGAUGUa -3' miRNA: 3'- aAUC-AGU--AUGUGGUGG-GGCGCUGUAg -5' |
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21157 | 5' | -53.1 | NC_004775.1 | + | 18645 | 0.67 | 0.62382 |
Target: 5'- -cGGUCuggACACCuACgCCCGCGAUg-- -3' miRNA: 3'- aaUCAGua-UGUGG-UG-GGGCGCUGuag -5' |
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21157 | 5' | -53.1 | NC_004775.1 | + | 808 | 0.66 | 0.681668 |
Target: 5'- gUGG-CAUGCGCCAgCUCUGUGcCAUCc -3' miRNA: 3'- aAUCaGUAUGUGGU-GGGGCGCuGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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