Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21158 | 3' | -52.7 | NC_004775.1 | + | 14034 | 0.66 | 0.779035 |
Target: 5'- uGGCGCaaugGCagaggCUACCugUCgCAUCGCCg -3' miRNA: 3'- gUCGCG----CGaaaa-GAUGG--AG-GUAGCGG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 4882 | 0.66 | 0.725044 |
Target: 5'- uGGCucccuGUGCUgcggcCUGCC-CCAUUGCCa -3' miRNA: 3'- gUCG-----CGCGAaaa--GAUGGaGGUAGCGG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 1275 | 0.68 | 0.657056 |
Target: 5'- uCAGCGCGCcuucugCUACUUCCcacaccagAUCGgCa -3' miRNA: 3'- -GUCGCGCGaaaa--GAUGGAGG--------UAGCgG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 18473 | 0.7 | 0.487974 |
Target: 5'- uGGCaGCGCUUggagaUCUACCgCCugagCGCCu -3' miRNA: 3'- gUCG-CGCGAAa----AGAUGGaGGua--GCGG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 32960 | 0.7 | 0.520611 |
Target: 5'- gCGGCGgccUGCUUcUCUACagUCCAUuCGCCa -3' miRNA: 3'- -GUCGC---GCGAAaAGAUGg-AGGUA-GCGG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 21782 | 0.7 | 0.531681 |
Target: 5'- uGGCGCuGCUUUUUcACCgCCGUCuGCUa -3' miRNA: 3'- gUCGCG-CGAAAAGaUGGaGGUAG-CGG- -5' |
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21158 | 3' | -52.7 | NC_004775.1 | + | 29532 | 1.13 | 0.000569 |
Target: 5'- aCAGCGCGCUUUUCUACCUCCAUCGCCg -3' miRNA: 3'- -GUCGCGCGAAAAGAUGGAGGUAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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