miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21160 5' -52.7 NC_004775.1 + 35440 0.67 0.677678
Target:  5'- aAGCGUCgUGCA-CAGGAcGCaGACAGGAa -3'
miRNA:   3'- -UCGCAG-GCGUaGUCUU-CGcCUGUUUU- -5'
21160 5' -52.7 NC_004775.1 + 39161 0.68 0.6078
Target:  5'- aAGCGUCUgauaguugaGCGggAGAAGCGG-CAGAAa -3'
miRNA:   3'- -UCGCAGG---------CGUagUCUUCGCCuGUUUU- -5'
21160 5' -52.7 NC_004775.1 + 32159 0.7 0.505165
Target:  5'- uGGCGUUCGCcgUgaaauucuGGAAGCGG-CAAAAg -3'
miRNA:   3'- -UCGCAGGCGuaG--------UCUUCGCCuGUUUU- -5'
21160 5' -52.7 NC_004775.1 + 26026 0.71 0.410693
Target:  5'- cGCGUCaCGCAgaUCAuaaauGGCGGGCAGAAc -3'
miRNA:   3'- uCGCAG-GCGU--AGUcu---UCGCCUGUUUU- -5'
21160 5' -52.7 NC_004775.1 + 35204 0.86 0.040942
Target:  5'- uGGCGUCCGCAUUgaAAGCGGACAGAu -3'
miRNA:   3'- -UCGCAGGCGUAGucUUCGCCUGUUUu -5'
21160 5' -52.7 NC_004775.1 + 35483 1.07 0.001448
Target:  5'- aAGCGUCCGCAUCAGAAGCGGACAAAAa -3'
miRNA:   3'- -UCGCAGGCGUAGUCUUCGCCUGUUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.