miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21162 5' -47.2 NC_004777.1 + 21855 0.73 0.62905
Target:  5'- cUGCCgcUGAGUUAUCCGCUGUg--- -3'
miRNA:   3'- uAUGGgaACUCAAUAGGCGAUAagua -5'
21162 5' -47.2 NC_004777.1 + 36163 0.71 0.737999
Target:  5'- -gACCCUUGAGUUGcUCGCUAa---- -3'
miRNA:   3'- uaUGGGAACUCAAUaGGCGAUaagua -5'
21162 5' -47.2 NC_004777.1 + 12662 0.66 0.954356
Target:  5'- -cACCCUUGuGcUGUCgCGCUugAUUCAc -3'
miRNA:   3'- uaUGGGAACuCaAUAG-GCGA--UAAGUa -5'
21162 5' -47.2 NC_004777.1 + 891 1.05 0.005969
Target:  5'- gAUACCCUUGAGUUAUCCGCUAUUCAUg -3'
miRNA:   3'- -UAUGGGAACUCAAUAGGCGAUAAGUA- -5'
21162 5' -47.2 NC_004777.1 + 26565 0.7 0.794607
Target:  5'- -cACCgUUGAGUUccacUCgGCUGUUCAUg -3'
miRNA:   3'- uaUGGgAACUCAAu---AGgCGAUAAGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.