Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21163 | 3' | -53.7 | NC_004777.1 | + | 25916 | 0.67 | 0.594472 |
Target: 5'- uAUGAGcAAgauugaaucugccCUUCAAgcGGCCAGCCGGGAc -3' miRNA: 3'- -UGCUCaUU-------------GGAGUU--UCGGUCGGCCUU- -5' |
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21163 | 3' | -53.7 | NC_004777.1 | + | 7156 | 1.05 | 0.001249 |
Target: 5'- cACGAGUAACCUCAAAGCCAGCCGGAAc -3' miRNA: 3'- -UGCUCAUUGGAGUUUCGGUCGGCCUU- -5' |
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21163 | 3' | -53.7 | NC_004777.1 | + | 3314 | 0.69 | 0.451154 |
Target: 5'- uGCGAGUAacACCgu-AAGCCAGCCa--- -3' miRNA: 3'- -UGCUCAU--UGGaguUUCGGUCGGccuu -5' |
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21163 | 3' | -53.7 | NC_004777.1 | + | 20392 | 0.66 | 0.642159 |
Target: 5'- cACGcuuGGUAGCCUgAGGuuUAGCCGGAAu -3' miRNA: 3'- -UGC---UCAUUGGAgUUUcgGUCGGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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