miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21168 3' -55.1 NC_004777.1 + 20409 0.66 0.583506
Target:  5'- aCGGUccgaaaAGCCuucacgcuugGUAGCCUgAGGUuuAGCCg -3'
miRNA:   3'- -GUCG------UCGG----------UAUCGGAgUCCAcuUCGG- -5'
21168 3' -55.1 NC_004777.1 + 2578 0.66 0.580086
Target:  5'- gCAGCAGCaCGUuuccaugcggugagAGCCUCAGGauucguGUCg -3'
miRNA:   3'- -GUCGUCG-GUA--------------UCGGAGUCCacuu--CGG- -5'
21168 3' -55.1 NC_004777.1 + 20910 0.67 0.494409
Target:  5'- uCAGCAGUCAggaGGCCaUCAauGGUGAuGaCCu -3'
miRNA:   3'- -GUCGUCGGUa--UCGG-AGU--CCACUuC-GG- -5'
21168 3' -55.1 NC_004777.1 + 21185 0.68 0.421863
Target:  5'- aAGCAGCacgAGCCUUAGucaGAGCCg -3'
miRNA:   3'- gUCGUCGguaUCGGAGUCcacUUCGG- -5'
21168 3' -55.1 NC_004777.1 + 29810 0.69 0.383391
Target:  5'- -uGCAGUCugguGCCUCAGG-GAAGUUc -3'
miRNA:   3'- guCGUCGGuau-CGGAGUCCaCUUCGG- -5'
21168 3' -55.1 NC_004777.1 + 25021 0.78 0.093469
Target:  5'- cCAGCAGCCuUAGCUUCAagaaugucauGGUG-AGCCg -3'
miRNA:   3'- -GUCGUCGGuAUCGGAGU----------CCACuUCGG- -5'
21168 3' -55.1 NC_004777.1 + 19458 1.12 0.000292
Target:  5'- gCAGCAGCCAUAGCCUCAGGUGAAGCCg -3'
miRNA:   3'- -GUCGUCGGUAUCGGAGUCCACUUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.