Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21169 | 3' | -42.8 | NC_004777.1 | + | 30865 | 0.66 | 0.999136 |
|
Target: 5'- uUCUU--AGCGAAGAACCCUaggUCAuuGAUg -3' miRNA: 3'- -GGAAauUCGUUUUUUGGGG---AGUu-CUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 25914 | 0.72 | 0.951426 |
|
Target: 5'- aCUaUGAGCAAGAuugaaucuGCCCUUCAAG-Cg -3' miRNA: 3'- gGAaAUUCGUUUUu-------UGGGGAGUUCuG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 25862 | 0.66 | 0.999136 |
|
Target: 5'- aCUcgcAGCGGAAGACCCCau--GACa -3' miRNA: 3'- gGAaauUCGUUUUUUGGGGaguuCUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 22861 | 0.67 | 0.997406 |
|
Target: 5'- aCUU--GGCGAAuuacAACCCUaagCAAGACg -3' miRNA: 3'- gGAAauUCGUUUu---UUGGGGa--GUUCUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 21851 | 1.13 | 0.007509 |
|
Target: 5'- uCCUUUAAGCAAAAAACCCCUCAAGACc -3' miRNA: 3'- -GGAAAUUCGUUUUUUGGGGAGUUCUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 21744 | 0.75 | 0.85736 |
|
Target: 5'- aCCgcUGAGCAAuaacuagcauAACCCCUUggGGCc -3' miRNA: 3'- -GGaaAUUCGUUuu--------UUGGGGAGuuCUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 21046 | 0.66 | 0.999473 |
|
Target: 5'- aCUgcuacGGUAAuuGAGACCaCUCAGGACa -3' miRNA: 3'- gGAaau--UCGUU--UUUUGGgGAGUUCUG- -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 12838 | 0.68 | 0.994484 |
|
Target: 5'- cCCUUcaucucgccUAAGCGAu-AACCCCacgccUCAAGAg -3' miRNA: 3'- -GGAA---------AUUCGUUuuUUGGGG-----AGUUCUg -5' |
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| 21169 | 3' | -42.8 | NC_004777.1 | + | 12296 | 0.67 | 0.997887 |
|
Target: 5'- aCUUgu-GCGuauuuGCCCCUCAcugaguGGACa -3' miRNA: 3'- gGAAauuCGUuuuu-UGGGGAGU------UCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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