Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21173 | 3' | -44 | NC_004777.1 | + | 28330 | 1.12 | 0.007016 |
Target: 5'- uCUGUCCAAGCGACUUUACGUAAAAGGu -3' miRNA: 3'- -GACAGGUUCGCUGAAAUGCAUUUUCC- -5' |
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21173 | 3' | -44 | NC_004777.1 | + | 12495 | 0.8 | 0.541038 |
Target: 5'- -cGUCCGAGUGACUUcggUGCGUAucuGGa -3' miRNA: 3'- gaCAGGUUCGCUGAA---AUGCAUuuuCC- -5' |
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21173 | 3' | -44 | NC_004777.1 | + | 28679 | 0.71 | 0.946701 |
Target: 5'- aCUGUCCAgccaucGGUGACUUaGCGacauccAGAAGGu -3' miRNA: 3'- -GACAGGU------UCGCUGAAaUGCa-----UUUUCC- -5' |
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21173 | 3' | -44 | NC_004777.1 | + | 9961 | 0.7 | 0.972036 |
Target: 5'- aUGUaCCAAGUGGCUgacUACGUGAc--- -3' miRNA: 3'- gACA-GGUUCGCUGAa--AUGCAUUuucc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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