miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21174 5' -47.3 NC_004777.1 + 24840 0.66 0.967996
Target:  5'- uCUCCGuaaguAGUUUCCuuc---CCAGCCa -3'
miRNA:   3'- -GAGGUuu---UCAAAGGuugacaGGUCGG- -5'
21174 5' -47.3 NC_004777.1 + 14653 0.7 0.827445
Target:  5'- gUCCAGAaucuuuucucgcaucAGUUcuucggUCUcGCUGUCCAGCUu -3'
miRNA:   3'- gAGGUUU---------------UCAA------AGGuUGACAGGUCGG- -5'
21174 5' -47.3 NC_004777.1 + 26090 0.74 0.635336
Target:  5'- -aCCAAuGGUgUCCAauaggcuugacuuugGCUGUUCAGCCu -3'
miRNA:   3'- gaGGUUuUCAaAGGU---------------UGACAGGUCGG- -5'
21174 5' -47.3 NC_004777.1 + 28695 1.15 0.001712
Target:  5'- cCUCCAAAAGUUUCCAACUGUCCAGCCa -3'
miRNA:   3'- -GAGGUUUUCAAAGGUUGACAGGUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.