miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21178 5' -43.4 NC_004777.1 + 27574 0.66 0.998776
Target:  5'- aCGCUugGCAGucaGUCGGucagcgucaaGAAUAA-CCUg -3'
miRNA:   3'- aGCGAugCGUU---UAGUC----------UUUAUUaGGA- -5'
21178 5' -43.4 NC_004777.1 + 28362 0.67 0.997144
Target:  5'- cCGCUAaugcccuuggucUGCGAGUUAcAGAUGGUCCa -3'
miRNA:   3'- aGCGAU------------GCGUUUAGUcUUUAUUAGGa -5'
21178 5' -43.4 NC_004777.1 + 7406 0.69 0.979895
Target:  5'- aCGCUggcaGCGCGGGUCuuuguuGGGAAUAGUCa- -3'
miRNA:   3'- aGCGA----UGCGUUUAG------UCUUUAUUAGga -5'
21178 5' -43.4 NC_004777.1 + 22817 0.69 0.982419
Target:  5'- gUCGCUACGUGGAUUAGc---GGUCUUg -3'
miRNA:   3'- -AGCGAUGCGUUUAGUCuuuaUUAGGA- -5'
21178 5' -43.4 NC_004777.1 + 32894 0.7 0.963115
Target:  5'- cUGCUGCGCAucAAUCuGAGGUAAacgcugagaacUCCg -3'
miRNA:   3'- aGCGAUGCGU--UUAGuCUUUAUU-----------AGGa -5'
21178 5' -43.4 NC_004777.1 + 7923 0.74 0.872158
Target:  5'- aCGCUGCGCGugguAUCAGAAA-GGUCg- -3'
miRNA:   3'- aGCGAUGCGUu---UAGUCUUUaUUAGga -5'
21178 5' -43.4 NC_004777.1 + 34797 1.11 0.008344
Target:  5'- gUCGCUACGCAAAUCAGAAAUAAUCCUa -3'
miRNA:   3'- -AGCGAUGCGUUUAGUCUUUAUUAGGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.