Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21181 | 5' | -60.7 | NC_004778.3 | + | 34162 | 0.66 | 0.635764 |
Target: 5'- cGGCUGCaagCACGCGUUagcgacggugcGGucGCCGCgGCg -3' miRNA: 3'- -CUGGCG---GUGCGCAA-----------CCucCGGCGaCG- -5' |
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21181 | 5' | -60.7 | NC_004778.3 | + | 94518 | 0.67 | 0.629741 |
Target: 5'- cGCCGCCGCGCacaaUGGccauGcgcuugcgguauaacGCCGCUGCc -3' miRNA: 3'- cUGGCGGUGCGca--ACCu---C---------------CGGCGACG- -5' |
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21181 | 5' | -60.7 | NC_004778.3 | + | 41206 | 0.66 | 0.685765 |
Target: 5'- cACCGUCGCacgugguuucGCGUUugacGGGcGGCCGCgccgGCa -3' miRNA: 3'- cUGGCGGUG----------CGCAA----CCU-CCGGCGa---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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