Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21184 | 5' | -55.9 | NC_004778.3 | + | 30719 | 0.67 | 0.785051 |
Target: 5'- uGugUGUGCAU-CUGCCGGagaaggagcacGAGCGCGa -3' miRNA: 3'- -CugGUACGUAcGACGGUU-----------CUCGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 30728 | 0.67 | 0.785051 |
Target: 5'- cGGCCGcgGCcgGCUGCCAucgaaAGuuCGCGUc -3' miRNA: 3'- -CUGGUa-CGuaCGACGGU-----UCucGCGCA- -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 53666 | 0.71 | 0.537802 |
Target: 5'- aGGCCGUGUuugcgcacAUGCuUGCCGccagcgccgaGGAGCGCGa -3' miRNA: 3'- -CUGGUACG--------UACG-ACGGU----------UCUCGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 62207 | 0.73 | 0.430354 |
Target: 5'- cGGCCucuUGCAUGUUGUCAGGcagcguuGCGCGUu -3' miRNA: 3'- -CUGGu--ACGUACGACGGUUCu------CGCGCA- -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 80560 | 0.66 | 0.821732 |
Target: 5'- uGCCGauacgcGCGUGCcGCCAAGGccguGCGCGa -3' miRNA: 3'- cUGGUa-----CGUACGaCGGUUCU----CGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 83845 | 0.67 | 0.765794 |
Target: 5'- cGGCUAUGCAgaagGCgcGCCAggcgcuggaGGAGCGCu- -3' miRNA: 3'- -CUGGUACGUa---CGa-CGGU---------UCUCGCGca -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 94533 | 0.66 | 0.86026 |
Target: 5'- uGGCCAUGCGcUugcgguauaacgccGCUGCCGAGGucgacguGCGCa- -3' miRNA: 3'- -CUGGUACGU-A--------------CGACGGUUCU-------CGCGca -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 98831 | 0.68 | 0.75597 |
Target: 5'- -----aGCAUGCccaaUGCCAcGGAGCGCGg -3' miRNA: 3'- cugguaCGUACG----ACGGU-UCUCGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 99305 | 0.71 | 0.537802 |
Target: 5'- cGGCCAUgGCuuuuGUGCUGUCAcugAGuGCGCGUg -3' miRNA: 3'- -CUGGUA-CG----UACGACGGU---UCuCGCGCA- -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 101435 | 0.67 | 0.803722 |
Target: 5'- cGCCAUGCGuUGCU-CgCAGGuGCGCGc -3' miRNA: 3'- cUGGUACGU-ACGAcG-GUUCuCGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 117358 | 0.67 | 0.794464 |
Target: 5'- aGCC--GCAUaGCUGCUAAGgcgGGCGCGg -3' miRNA: 3'- cUGGuaCGUA-CGACGGUUC---UCGCGCa -5' |
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21184 | 5' | -55.9 | NC_004778.3 | + | 127153 | 1.07 | 0.002617 |
Target: 5'- cGACCAUGCAUGCUGCCAAGAGCGCGUc -3' miRNA: 3'- -CUGGUACGUACGACGGUUCUCGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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