Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21185 | 3' | -59.6 | NC_004778.3 | + | 120051 | 0.66 | 0.67927 |
Target: 5'- uGCGCGcGugGUGaaauuGGucGCCCAUCauGGGCAg -3' miRNA: 3'- uUGCGC-CugCAC-----CC--UGGGUAG--UCCGU- -5' |
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21185 | 3' | -59.6 | NC_004778.3 | + | 105822 | 0.67 | 0.648858 |
Target: 5'- cAUGCGGACGUGGGACgUCGUU--GCc -3' miRNA: 3'- uUGCGCCUGCACCCUG-GGUAGucCGu -5' |
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21185 | 3' | -59.6 | NC_004778.3 | + | 18239 | 0.69 | 0.534914 |
Target: 5'- --gGCGGAuuugguuuuggcgcUGUGGGACCCAUuUGGGUAc -3' miRNA: 3'- uugCGCCU--------------GCACCCUGGGUA-GUCCGU- -5' |
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21185 | 3' | -59.6 | NC_004778.3 | + | 80652 | 0.7 | 0.470584 |
Target: 5'- gGACGUGGGCGUGGGAgaCGgu-GGCGc -3' miRNA: 3'- -UUGCGCCUGCACCCUggGUaguCCGU- -5' |
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21185 | 3' | -59.6 | NC_004778.3 | + | 58418 | 0.77 | 0.189615 |
Target: 5'- uACGCGGuucCGUGGGACUguCGUCGGGUAa -3' miRNA: 3'- uUGCGCCu--GCACCCUGG--GUAGUCCGU- -5' |
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21185 | 3' | -59.6 | NC_004778.3 | + | 125365 | 1.06 | 0.001703 |
Target: 5'- uAACGCGGACGUGGGACCCAUCAGGCAg -3' miRNA: 3'- -UUGCGCCUGCACCCUGGGUAGUCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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