Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21186 | 5' | -52.6 | NC_004778.3 | + | 6804 | 0.66 | 0.965702 |
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Target: 5'- -gGGUGUUUGgucacGUCGCGCGGAAa- -3' miRNA: 3'- cgUCACAAGUacu--CGGCGCGCCUUau -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 42321 | 0.66 | 0.958504 |
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Target: 5'- uGCGaUGUuugccaucUUAUGucGCCGCGCGGAAc- -3' miRNA: 3'- -CGUcACA--------AGUACu-CGGCGCGCCUUau -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 112269 | 0.66 | 0.950333 |
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Target: 5'- uGCAGUcg-CGgu-GcCCGCGCGGAAUAg -3' miRNA: 3'- -CGUCAcaaGUacuC-GGCGCGCCUUAU- -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 26808 | 0.68 | 0.907392 |
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Target: 5'- aUAGagGUUgcUGGGCCGCGCGGGu-- -3' miRNA: 3'- cGUCa-CAAguACUCGGCGCGCCUuau -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 12418 | 0.69 | 0.887032 |
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Target: 5'- uCGGUGUUCGUGGucguGCCGgugGCGGggUc -3' miRNA: 3'- cGUCACAAGUACU----CGGCg--CGCCuuAu -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 60230 | 0.7 | 0.804173 |
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Target: 5'- aUAGUGUcuuaUCAUGuuGCUGCGCGGGc-- -3' miRNA: 3'- cGUCACA----AGUACu-CGGCGCGCCUuau -5' |
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| 21186 | 5' | -52.6 | NC_004778.3 | + | 122669 | 1.05 | 0.007944 |
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Target: 5'- gGCAGUGUUCAUGAGCCGCGCGGAAg- -3' miRNA: 3'- -CGUCACAAGUACUCGGCGCGCCUUau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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