Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21191 | 5' | -50 | NC_004778.3 | + | 24973 | 0.66 | 0.996292 |
Target: 5'- aCAUUUGACUUUGUCGCuaCGGcCGg-- -3' miRNA: 3'- gGUGAAUUGGAACAGCG--GCCaGUacc -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 45617 | 0.66 | 0.995652 |
Target: 5'- gCCGCgc--CCUcGUCGCCGGcguugaguUUGUGGu -3' miRNA: 3'- -GGUGaauuGGAaCAGCGGCC--------AGUACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 121328 | 0.66 | 0.994925 |
Target: 5'- -aGCUgcGCCgUGUCggcgauaaGCgGGUCAUGGu -3' miRNA: 3'- ggUGAauUGGaACAG--------CGgCCAGUACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 7001 | 0.67 | 0.994101 |
Target: 5'- aCCGuCUcGAUCUUGUCGCgcauaucgucgCGGUCAUa- -3' miRNA: 3'- -GGU-GAaUUGGAACAGCG-----------GCCAGUAcc -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 49287 | 0.67 | 0.993835 |
Target: 5'- aCCGCU----CUUGUCGCCgaacagcgcggcgcGGUCAUuGGa -3' miRNA: 3'- -GGUGAauugGAACAGCGG--------------CCAGUA-CC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 34560 | 0.67 | 0.992134 |
Target: 5'- aCACgc-ACCgaaUGUCGCCGcUCAUGu -3' miRNA: 3'- gGUGaauUGGa--ACAGCGGCcAGUACc -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 35204 | 0.68 | 0.988243 |
Target: 5'- -aGCUUGACUUUGgcgccCGCCGuGUCGUa- -3' miRNA: 3'- ggUGAAUUGGAACa----GCGGC-CAGUAcc -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 59609 | 0.69 | 0.97617 |
Target: 5'- gCCACUUGGCCgaaaaccgGUCGuCCGGauaCAcuUGGu -3' miRNA: 3'- -GGUGAAUUGGaa------CAGC-GGCCa--GU--ACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 32463 | 0.69 | 0.967512 |
Target: 5'- gCCGCU--GCg-UGUCGCCuuugcaccagcGGUCGUGGc -3' miRNA: 3'- -GGUGAauUGgaACAGCGG-----------CCAGUACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 44991 | 0.69 | 0.967512 |
Target: 5'- aCCGC--GACCa-GUCGCUGGUUuUGGa -3' miRNA: 3'- -GGUGaaUUGGaaCAGCGGCCAGuACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 74691 | 0.7 | 0.964185 |
Target: 5'- cUCACUU--CCUUGUCGCUcGUCuccuUGGa -3' miRNA: 3'- -GGUGAAuuGGAACAGCGGcCAGu---ACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 23281 | 0.7 | 0.964185 |
Target: 5'- gUACUUGACCguguUGUUGCCGGgc--GGa -3' miRNA: 3'- gGUGAAUUGGa---ACAGCGGCCaguaCC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 49118 | 0.7 | 0.952795 |
Target: 5'- uCCACgUAAUCggGUugCGCCGGUCccacGUGGa -3' miRNA: 3'- -GGUGaAUUGGaaCA--GCGGCCAG----UACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 13886 | 0.72 | 0.917482 |
Target: 5'- gUC-CUUGGCCagcgcuaugagaUUGUCGCCGcUCAUGGu -3' miRNA: 3'- -GGuGAAUUGG------------AACAGCGGCcAGUACC- -5' |
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21191 | 5' | -50 | NC_004778.3 | + | 117126 | 1.13 | 0.00651 |
Target: 5'- gCCACUUAACCUUGUCGCCGGUCAUGGc -3' miRNA: 3'- -GGUGAAUUGGAACAGCGGCCAGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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