Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21194 | 3' | -50.7 | NC_004778.3 | + | 122774 | 0.66 | 0.985946 |
Target: 5'- aUGGACACcauguuugagggcGUGCACCCCaauccgcUGUUGGCc -3' miRNA: 3'- aAUUUGUGau-----------CGCGUGGGG-------ACAACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 103387 | 0.66 | 0.983471 |
Target: 5'- --uGGCGCUGGCGC-CCUCgucgUGuUUGGCg -3' miRNA: 3'- aauUUGUGAUCGCGuGGGG----AC-AACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 105145 | 0.66 | 0.983471 |
Target: 5'- aUUGGGCGCcgcGGCGCGCU--UGUUGGCu -3' miRNA: 3'- -AAUUUGUGa--UCGCGUGGggACAACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 87893 | 0.66 | 0.981352 |
Target: 5'- -gGAAUACUuGCGCACCUgU-UUGACc -3' miRNA: 3'- aaUUUGUGAuCGCGUGGGgAcAACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 72361 | 0.66 | 0.979036 |
Target: 5'- gUAAACuGCUcgcuuguacGGCGCAUUCCUGgucagcgUGACa -3' miRNA: 3'- aAUUUG-UGA---------UCGCGUGGGGACa------ACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 9903 | 0.66 | 0.979036 |
Target: 5'- --uAGCGCUGGCGCAUUuugggCUUGaUGACg -3' miRNA: 3'- aauUUGUGAUCGCGUGG-----GGACaACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 47734 | 0.67 | 0.973775 |
Target: 5'- ----cCGCUGGCGCAagCCUUGU-GGCg -3' miRNA: 3'- aauuuGUGAUCGCGUg-GGGACAaCUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 81292 | 0.68 | 0.956562 |
Target: 5'- aUAGACACgcacggcGGCGCggauuGCCCCUGUUu-- -3' miRNA: 3'- aAUUUGUGa------UCGCG-----UGGGGACAAcug -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 36642 | 0.69 | 0.90908 |
Target: 5'- --cGGCGCUGGCGCGCUCCguuccgGUguGCa -3' miRNA: 3'- aauUUGUGAUCGCGUGGGGa-----CAacUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 37725 | 0.7 | 0.888359 |
Target: 5'- -gAAGCACUcguacuguuGGCGCAgCCCgcuuauuucaaUGUUGGCa -3' miRNA: 3'- aaUUUGUGA---------UCGCGUgGGG-----------ACAACUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 6826 | 0.75 | 0.635522 |
Target: 5'- -gAAACACcacgacgGGCGC-CCCCUGUgugGACg -3' miRNA: 3'- aaUUUGUGa------UCGCGuGGGGACAa--CUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 19393 | 0.8 | 0.380406 |
Target: 5'- ---cGC-CUGGCGCACCCCUGUuacgGGCa -3' miRNA: 3'- aauuUGuGAUCGCGUGGGGACAa---CUG- -5' |
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21194 | 3' | -50.7 | NC_004778.3 | + | 113078 | 1.09 | 0.006121 |
Target: 5'- aUUAAACACUAGCGCACCCCUGUUGACg -3' miRNA: 3'- -AAUUUGUGAUCGCGUGGGGACAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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