Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21194 | 5' | -48 | NC_004778.3 | + | 124372 | 0.66 | 0.998488 |
Target: 5'- ---cCGGCGGCGGCUGaCGgGGgUGUUg -3' miRNA: 3'- uuuaGUUGUCGCUGAC-GCgUCaAUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 118126 | 0.66 | 0.998488 |
Target: 5'- aAAAUCGGCGGCGGC-GC-CGGUUc-- -3' miRNA: 3'- -UUUAGUUGUCGCUGaCGcGUCAAuag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 71483 | 0.66 | 0.997781 |
Target: 5'- -uGUCGGCGGgGGCguugGCGgAGgaGUCg -3' miRNA: 3'- uuUAGUUGUCgCUGa---CGCgUCaaUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 81850 | 0.66 | 0.997781 |
Target: 5'- cGGAUCGggaGCGGCGGCgguagaccaugGUGCuagAGUUGUCa -3' miRNA: 3'- -UUUAGU---UGUCGCUGa----------CGCG---UCAAUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 83824 | 0.66 | 0.997781 |
Target: 5'- --cUCAACGccGCGACgaggGCGCGGcUAUg -3' miRNA: 3'- uuuAGUUGU--CGCUGa---CGCGUCaAUAg -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 95254 | 0.66 | 0.99774 |
Target: 5'- ---aCAACAGCcacagcacguccuGcauGCUGCGCAGcUUGUCg -3' miRNA: 3'- uuuaGUUGUCG-------------C---UGACGCGUC-AAUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 31769 | 0.66 | 0.997335 |
Target: 5'- --uUCAAguGUGAUUGCGCA---AUCa -3' miRNA: 3'- uuuAGUUguCGCUGACGCGUcaaUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 12094 | 0.66 | 0.997335 |
Target: 5'- ---gCAGCuauGCGGCUGCGCaAGcaAUCu -3' miRNA: 3'- uuuaGUUGu--CGCUGACGCG-UCaaUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 63725 | 0.67 | 0.99553 |
Target: 5'- cGGGUCgAACAGCGACcGCGCGa----- -3' miRNA: 3'- -UUUAG-UUGUCGCUGaCGCGUcaauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 88625 | 0.67 | 0.99553 |
Target: 5'- ---gCAGCAGCGuCUGCGCGu----- -3' miRNA: 3'- uuuaGUUGUCGCuGACGCGUcaauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 90507 | 0.67 | 0.99553 |
Target: 5'- ---gCGGuCAGUGGCcGCGCAGUUAc- -3' miRNA: 3'- uuuaGUU-GUCGCUGaCGCGUCAAUag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 106619 | 0.67 | 0.99553 |
Target: 5'- -cAUUAACGGCGugucgcaaacGCUGCGCAGc---- -3' miRNA: 3'- uuUAGUUGUCGC----------UGACGCGUCaauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 34164 | 0.67 | 0.993847 |
Target: 5'- ---cCGGCGGCGacaaGCUGCGCAGc---- -3' miRNA: 3'- uuuaGUUGUCGC----UGACGCGUCaauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 119113 | 0.67 | 0.992833 |
Target: 5'- cAAGUUGACcGCGACgcGCGCAGUa--- -3' miRNA: 3'- -UUUAGUUGuCGCUGa-CGCGUCAauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 122844 | 0.68 | 0.989852 |
Target: 5'- ---cCAACAGCGGCuauaaaaUGCGUcguuugucuaaaaaGGUUAUCg -3' miRNA: 3'- uuuaGUUGUCGCUG-------ACGCG--------------UCAAUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 985 | 0.68 | 0.98561 |
Target: 5'- cGGcgCAGCAGCGGCUccaacGCGUGGcgGUCc -3' miRNA: 3'- -UUuaGUUGUCGCUGA-----CGCGUCaaUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 54130 | 0.7 | 0.970794 |
Target: 5'- ---cCGACAGUGACcuuguUGCGCAGUa--- -3' miRNA: 3'- uuuaGUUGUCGCUG-----ACGCGUCAauag -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 115095 | 0.7 | 0.964018 |
Target: 5'- --uUCGuCAGCGAUgccGCGCGcGUUAUCg -3' miRNA: 3'- uuuAGUuGUCGCUGa--CGCGU-CAAUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 32778 | 0.7 | 0.960247 |
Target: 5'- uGAAgu-GCAGCGACUGCGCGaacauGgaGUCg -3' miRNA: 3'- -UUUaguUGUCGCUGACGCGU-----CaaUAG- -5' |
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21194 | 5' | -48 | NC_004778.3 | + | 119363 | 0.71 | 0.947328 |
Target: 5'- -uGUCAAUAGUGAgCUgGCGCAGUUuacugcGUCg -3' miRNA: 3'- uuUAGUUGUCGCU-GA-CGCGUCAA------UAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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