Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21197 | 5' | -59.4 | NC_004778.3 | + | 57130 | 0.66 | 0.711128 |
Target: 5'- uGGGCCCCGACGgaACGGGaGAcGUGc -3' miRNA: 3'- uUCUGGGGUUGCggUGCCCgCUuCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 59937 | 0.66 | 0.711128 |
Target: 5'- cGGACCCCAuCGCCGCugaaauGGCGcuuGUGu -3' miRNA: 3'- uUCUGGGGUuGCGGUGc-----CCGCuu-CGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 98358 | 0.66 | 0.701177 |
Target: 5'- cAGACgCgCAccGCGCC-CGGGCGcAGCa -3' miRNA: 3'- uUCUGgG-GU--UGCGGuGCCCGCuUCGc -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 99016 | 0.66 | 0.701177 |
Target: 5'- cGGGugCCgCAACGCC-CGGcaacGCGucGCGg -3' miRNA: 3'- -UUCugGG-GUUGCGGuGCC----CGCuuCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 90241 | 0.67 | 0.671014 |
Target: 5'- cGAGuCgCUUAGCGCCugcaugaccguaGCGGGCGAcGCGg -3' miRNA: 3'- -UUCuG-GGGUUGCGG------------UGCCCGCUuCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 93672 | 0.67 | 0.64057 |
Target: 5'- cAGACCgCCAAccaaguugucCGCCAgcuCGGGCGAuugguacaaAGCGu -3' miRNA: 3'- uUCUGG-GGUU----------GCGGU---GCCCGCU---------UCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 10820 | 0.68 | 0.599919 |
Target: 5'- cGAGGCUUauuuaaaaAGCGCCugGGGCGcguuGCGa -3' miRNA: 3'- -UUCUGGGg-------UUGCGGugCCCGCuu--CGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 26063 | 0.7 | 0.500811 |
Target: 5'- gAGGGCgCCAGCGCCACcaaaGGCGAucugauGCa -3' miRNA: 3'- -UUCUGgGGUUGCGGUGc---CCGCUu-----CGc -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 54610 | 0.7 | 0.491262 |
Target: 5'- -cGGCgCCCAcCGaCUACGGGCGcaAGGCGg -3' miRNA: 3'- uuCUG-GGGUuGC-GGUGCCCGC--UUCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 22572 | 0.7 | 0.491262 |
Target: 5'- -cGGCUUUAACGCCA-GGGCGucGGCGg -3' miRNA: 3'- uuCUGGGGUUGCGGUgCCCGCu-UCGC- -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 98836 | 0.71 | 0.435886 |
Target: 5'- ----gCCCAAUGCCACGGaGCGcGGCa -3' miRNA: 3'- uucugGGGUUGCGGUGCC-CGCuUCGc -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 52829 | 0.73 | 0.307751 |
Target: 5'- uGGACCCCGACGCCAUGccGGCGcugauuguGCa -3' miRNA: 3'- uUCUGGGGUUGCGGUGC--CCGCuu------CGc -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 114358 | 0.85 | 0.053109 |
Target: 5'- -uGACCCUGcGCGCCGCGGGCGAGGCc -3' miRNA: 3'- uuCUGGGGU-UGCGGUGCCCGCUUCGc -5' |
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21197 | 5' | -59.4 | NC_004778.3 | + | 107069 | 1.07 | 0.001583 |
Target: 5'- gAAGACCCCAACGCCACGGGCGAAGCGa -3' miRNA: 3'- -UUCUGGGGUUGCGGUGCCCGCUUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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