Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21198 | 3' | -54.1 | NC_004778.3 | + | 56173 | 0.66 | 0.941112 |
Target: 5'- cGCUGGGcGCguuGGGCgaucGCACGcccuGGUCGUAa -3' miRNA: 3'- -UGACCC-CG---CCUGa---UGUGCu---UUAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 98910 | 0.66 | 0.9257 |
Target: 5'- gUUGGGGCGGcCUuuaACGCGcagaccGAUCGUg -3' miRNA: 3'- uGACCCCGCCuGA---UGUGCu-----UUAGCGu -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 30649 | 0.67 | 0.914169 |
Target: 5'- --cGGGGuCGGACcGCGUGuuGUCGCGu -3' miRNA: 3'- ugaCCCC-GCCUGaUGUGCuuUAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 63935 | 0.67 | 0.895004 |
Target: 5'- gACUGcGGGUGcuGACUgaauACGCGAAG-CGCAc -3' miRNA: 3'- -UGAC-CCCGC--CUGA----UGUGCUUUaGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 44400 | 0.68 | 0.881014 |
Target: 5'- --cGucGCaGACUACACGAGAUUGCAc -3' miRNA: 3'- ugaCccCGcCUGAUGUGCUUUAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 75151 | 0.69 | 0.807258 |
Target: 5'- cGCcGGGGCGGGCUcACugGc---CGCAg -3' miRNA: 3'- -UGaCCCCGCCUGA-UGugCuuuaGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 118422 | 0.69 | 0.798117 |
Target: 5'- uUUGGGuGCGGGUUGCACGGcgcgccuguAUCGCAa -3' miRNA: 3'- uGACCC-CGCCUGAUGUGCUu--------UAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 125345 | 0.7 | 0.740286 |
Target: 5'- cGCUGGGuauaaguaCGGGCUGCGCGAucUUGUAa -3' miRNA: 3'- -UGACCCc-------GCCUGAUGUGCUuuAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 23141 | 0.71 | 0.709896 |
Target: 5'- gGCUGGGGCuGcGGCggGCACGGgcgcugacGAUUGCAg -3' miRNA: 3'- -UGACCCCG-C-CUGa-UGUGCU--------UUAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 9011 | 0.73 | 0.605515 |
Target: 5'- --gGGcGGCGGGCUGCAUuu-GUCGCAg -3' miRNA: 3'- ugaCC-CCGCCUGAUGUGcuuUAGCGU- -5' |
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21198 | 3' | -54.1 | NC_004778.3 | + | 106518 | 1.08 | 0.004092 |
Target: 5'- aACUGGGGCGGACUACACGAAAUCGCAc -3' miRNA: 3'- -UGACCCCGCCUGAUGUGCUUUAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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