Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21198 | 5' | -52.4 | NC_004778.3 | + | 32832 | 0.66 | 0.953896 |
Target: 5'- ----aGUUUCGgaCGCGGCuGGCCUa -3' miRNA: 3'- aaaagUAAAGCaaGCGUCGcCCGGGc -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 96345 | 0.67 | 0.949643 |
Target: 5'- -gUUCAUguaCGUguagcgCgGCAGCGGGCCg- -3' miRNA: 3'- aaAAGUAaa-GCAa-----G-CGUCGCCCGGgc -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 118774 | 0.67 | 0.945138 |
Target: 5'- ---aCGcUUCGggCGCGGCGcGGCCgGc -3' miRNA: 3'- aaaaGUaAAGCaaGCGUCGC-CCGGgC- -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 46691 | 0.67 | 0.930073 |
Target: 5'- -cUUCGgcgUCGgaauUCGCGGCGGGgggaCCGu -3' miRNA: 3'- aaAAGUaa-AGCa---AGCGUCGCCCg---GGC- -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 107142 | 0.68 | 0.899756 |
Target: 5'- ---cCAUgUCGUUgGUGGCGGGCgCGc -3' miRNA: 3'- aaaaGUAaAGCAAgCGUCGCCCGgGC- -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 91299 | 0.7 | 0.846849 |
Target: 5'- --gUCGag-CGg-CGCAGCGGGCCUGc -3' miRNA: 3'- aaaAGUaaaGCaaGCGUCGCCCGGGC- -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 14753 | 0.7 | 0.838383 |
Target: 5'- -------cUCGUUauauaGCAGCGGGCCUa -3' miRNA: 3'- aaaaguaaAGCAAg----CGUCGCCCGGGc -5' |
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21198 | 5' | -52.4 | NC_004778.3 | + | 106555 | 1.07 | 0.006811 |
Target: 5'- aUUUUCAUUUCGUUCGCAGCGGGCCCGu -3' miRNA: 3'- -AAAAGUAAAGCAAGCGUCGCCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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