Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21201 | 3' | -53.1 | NC_004778.3 | + | 91280 | 0.66 | 0.967553 |
Target: 5'- gCGCggguagCGugGa--GGUGuCGAGCGGCg -3' miRNA: 3'- aGCGaa----GCugCgaaCCAC-GCUUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 76365 | 0.66 | 0.967553 |
Target: 5'- gUUGCUgcggCGGCGgUUGcUGCG-GCGGCg -3' miRNA: 3'- -AGCGAa---GCUGCgAACcACGCuUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 76335 | 0.66 | 0.967553 |
Target: 5'- gUUGCUguggCGGCGgUUGcUGCG-GCGGCg -3' miRNA: 3'- -AGCGAa---GCUGCgAACcACGCuUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 46982 | 0.66 | 0.964262 |
Target: 5'- gCGCUcacgcCGACGCgcuuGUGCGAAucauCGACg -3' miRNA: 3'- aGCGAa----GCUGCGaac-CACGCUU----GCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 109958 | 0.66 | 0.964262 |
Target: 5'- uUUGCacgUCGGCGCgcccauuacGUGCG-ACGACa -3' miRNA: 3'- -AGCGa--AGCUGCGaac------CACGCuUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 10244 | 0.67 | 0.948775 |
Target: 5'- cUGCUgaacugcgUGugGCUUGGcGUGGACGGg -3' miRNA: 3'- aGCGAa-------GCugCGAACCaCGCUUGCUg -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 118774 | 0.67 | 0.948775 |
Target: 5'- aCGCUUCgGGCGC--GGcGCGGcCGGCg -3' miRNA: 3'- aGCGAAG-CUGCGaaCCaCGCUuGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 40744 | 0.67 | 0.948775 |
Target: 5'- gCGCacgCGcCGCUUGcUGCGcGGCGACa -3' miRNA: 3'- aGCGaa-GCuGCGAACcACGC-UUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 53293 | 0.67 | 0.939576 |
Target: 5'- -aGCagCGACGCUcuUGaGUGCGAA-GACc -3' miRNA: 3'- agCGaaGCUGCGA--AC-CACGCUUgCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 107267 | 0.67 | 0.939576 |
Target: 5'- -gGCUUCGGCGuUUUGcGUGCaauuaAACGACc -3' miRNA: 3'- agCGAAGCUGC-GAAC-CACGc----UUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 104169 | 0.67 | 0.931498 |
Target: 5'- gCGUUUUGcaaaguggagauuauGCGC-UGGgagGCGGGCGACg -3' miRNA: 3'- aGCGAAGC---------------UGCGaACCa--CGCUUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 71676 | 0.67 | 0.9239 |
Target: 5'- aCGUgu-GGCGCUUGGcgcgGCGGGCGuCg -3' miRNA: 3'- aGCGaagCUGCGAACCa---CGCUUGCuG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 16956 | 0.68 | 0.917583 |
Target: 5'- -gGCUcgaguucUCGGcCGC-UGGcGCGAGCGACu -3' miRNA: 3'- agCGA-------AGCU-GCGaACCaCGCUUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 98184 | 0.68 | 0.912188 |
Target: 5'- aCGCaaguuucUCGGCGCgaGGUGCGcGCGcCa -3' miRNA: 3'- aGCGa------AGCUGCGaaCCACGCuUGCuG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 40516 | 0.68 | 0.902098 |
Target: 5'- gCGCUUCGACGCc-GGcGCGucgcauucauuggcGCGACu -3' miRNA: 3'- aGCGAAGCUGCGaaCCaCGCu-------------UGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 99918 | 0.68 | 0.899477 |
Target: 5'- gUGCgaguauugCGGCGUUguaauuaaaaacUGGUGCGAagACGACa -3' miRNA: 3'- aGCGaa------GCUGCGA------------ACCACGCU--UGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 32922 | 0.69 | 0.885787 |
Target: 5'- cCGCa--GACGCgaaacacugugUUGG-GCGGACGACa -3' miRNA: 3'- aGCGaagCUGCG-----------AACCaCGCUUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 96773 | 0.69 | 0.863489 |
Target: 5'- -aGCUgccgCGGCGCgUGGUGCGuAAUGuGCa -3' miRNA: 3'- agCGAa---GCUGCGaACCACGC-UUGC-UG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 117399 | 0.7 | 0.829852 |
Target: 5'- aUGUUUCGAgGCcgUGGUGUccgacaaGAGCGGCu -3' miRNA: 3'- aGCGAAGCUgCGa-ACCACG-------CUUGCUG- -5' |
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21201 | 3' | -53.1 | NC_004778.3 | + | 82657 | 0.71 | 0.785526 |
Target: 5'- -aGCggacUUGGCGCUUGGcGCGGGCGcCa -3' miRNA: 3'- agCGa---AGCUGCGAACCaCGCUUGCuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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