Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21203 | 3' | -53.4 | NC_004778.3 | + | 80134 | 0.66 | 0.952795 |
Target: 5'- -aGUCGCGcGAcgGGaCGGGaCAcUGUUUCACg -3' miRNA: 3'- cgUAGCGC-CU--CC-GUCC-GU-ACAAAGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 910 | 0.66 | 0.952795 |
Target: 5'- gGCGgagGCGGAGGCggAGGUAUGcc-CGCc -3' miRNA: 3'- -CGUag-CGCCUCCG--UCCGUACaaaGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 829 | 0.66 | 0.952795 |
Target: 5'- gGCGgagGCGGAGGCggAGGUAUGcc-CGCc -3' miRNA: 3'- -CGUag-CGCCUCCG--UCCGUACaaaGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 88360 | 0.66 | 0.948512 |
Target: 5'- uGCGUCGUGGuGGUguuGGGCAccgcgcUGgacgCGCg -3' miRNA: 3'- -CGUAGCGCCuCCG---UCCGU------ACaaa-GUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 44155 | 0.66 | 0.934149 |
Target: 5'- gGCGuUUGCGGcGGCGacGGCAUGga-CGCg -3' miRNA: 3'- -CGU-AGCGCCuCCGU--CCGUACaaaGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 44125 | 0.66 | 0.934149 |
Target: 5'- gGCGuUUGCGGcGGCGacGGCAUGga-CGCg -3' miRNA: 3'- -CGU-AGCGCCuCCGU--CCGUACaaaGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 43225 | 0.7 | 0.792392 |
Target: 5'- aGUAUCGaGGAGGCGGGCGaaacaaacaUGag-CACg -3' miRNA: 3'- -CGUAGCgCCUCCGUCCGU---------ACaaaGUG- -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 25652 | 0.75 | 0.523997 |
Target: 5'- aGCuugCGCGGGGGCcggcGGCGUGUgggUCAa -3' miRNA: 3'- -CGua-GCGCCUCCGu---CCGUACAa--AGUg -5' |
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21203 | 3' | -53.4 | NC_004778.3 | + | 100177 | 1.12 | 0.002243 |
Target: 5'- gGCAUCGCGGAGGCAGGCAUGUUUCACa -3' miRNA: 3'- -CGUAGCGCCUCCGUCCGUACAAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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