Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21204 | 3' | -52.2 | NC_004778.3 | + | 94733 | 0.75 | 0.584621 |
Target: 5'- -cCAGCACGGGCgucuGGCuGCUGGcCGAg -3' miRNA: 3'- auGUCGUGCCCGaau-UUG-CGAUC-GCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 35844 | 0.77 | 0.460924 |
Target: 5'- cACAGCAUGGGUguUUGGugGCUuuuGGCGGg -3' miRNA: 3'- aUGUCGUGCCCG--AAUUugCGA---UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 98428 | 1.08 | 0.005324 |
Target: 5'- gUACAGCACGGGCUUAAACGCUAGCGAg -3' miRNA: 3'- -AUGUCGUGCCCGAAUUUGCGAUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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