Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21204 | 5' | -48.9 | NC_004778.3 | + | 60491 | 0.66 | 0.998395 |
Target: 5'- gCUCaGCUGcc---GCGCCuCGUUGAUGc -3' miRNA: 3'- aGAG-CGAUuuaaaCGCGG-GCAACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 29420 | 0.66 | 0.998203 |
Target: 5'- gCUCGUUAAA---GCGCUCGUuuugugcaauccugcUGGCGu -3' miRNA: 3'- aGAGCGAUUUaaaCGCGGGCA---------------ACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 22813 | 0.66 | 0.998065 |
Target: 5'- gCUCGCUGcgcagcGUUUGCGacacgCCGUuaaUGGCGu -3' miRNA: 3'- aGAGCGAUu-----UAAACGCg----GGCA---ACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 114337 | 0.66 | 0.996545 |
Target: 5'- gCUCGCUuuuGUUgagcgguuugacccUGCGCgCCGcgGGCGa -3' miRNA: 3'- aGAGCGAuu-UAA--------------ACGCG-GGCaaCUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 84378 | 0.67 | 0.994668 |
Target: 5'- cUCgcgCGCUGAugguaGCGCUCGUcGGCGc -3' miRNA: 3'- -AGa--GCGAUUuaaa-CGCGGGCAaCUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 91780 | 0.68 | 0.991693 |
Target: 5'- -aUCGCUucuUUaGCGCCCGUgcgcaucguguUGACa -3' miRNA: 3'- agAGCGAuuuAAaCGCGGGCA-----------ACUGc -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 55982 | 0.68 | 0.991575 |
Target: 5'- -gUCGCgcucggacagugGCGCCgCGUUGGCGu -3' miRNA: 3'- agAGCGauuuaaa-----CGCGG-GCAACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 51283 | 0.68 | 0.989069 |
Target: 5'- cUUUGCUGGAcaUGCGCCCGagGuCGu -3' miRNA: 3'- aGAGCGAUUUaaACGCGGGCaaCuGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 124087 | 0.68 | 0.98584 |
Target: 5'- --gCGCUGA---UGCGCgCgGUUGGCGg -3' miRNA: 3'- agaGCGAUUuaaACGCG-GgCAACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 8405 | 0.69 | 0.979691 |
Target: 5'- cCUUGCUAAAUgaaUGCGgCCGcauagagcaaaUUGACGc -3' miRNA: 3'- aGAGCGAUUUAa--ACGCgGGC-----------AACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 33558 | 0.69 | 0.97461 |
Target: 5'- --aCGCUGAucgUGUGUCCGUUGuCGc -3' miRNA: 3'- agaGCGAUUuaaACGCGGGCAACuGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 51183 | 0.7 | 0.965342 |
Target: 5'- aUUUCGUUAAug--GCGCCCaaacguaucgaGUUGGCGg -3' miRNA: 3'- -AGAGCGAUUuaaaCGCGGG-----------CAACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 60060 | 0.71 | 0.949609 |
Target: 5'- gCUCGUcAGA--UGCGUCCGUgGACGa -3' miRNA: 3'- aGAGCGaUUUaaACGCGGGCAaCUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 40433 | 0.72 | 0.924084 |
Target: 5'- --aCGCagUAAAUgaGcCGCCCGUUGACGa -3' miRNA: 3'- agaGCG--AUUUAaaC-GCGGGCAACUGC- -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 89815 | 0.74 | 0.861499 |
Target: 5'- gCUCGCUGGAacgGUGCCCGccgGACu -3' miRNA: 3'- aGAGCGAUUUaaaCGCGGGCaa-CUGc -5' |
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21204 | 5' | -48.9 | NC_004778.3 | + | 98462 | 1.1 | 0.010301 |
Target: 5'- uUCUCGCUAAAUUUGCGCCCGUUGACGu -3' miRNA: 3'- -AGAGCGAUUUAAACGCGGGCAACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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