Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21205 | 5' | -50.6 | NC_004778.3 | + | 65157 | 0.66 | 0.988904 |
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Target: 5'- -aUGGCGggcgaugacccGGACaCCGCGUUUGGc--- -3' miRNA: 3'- caACCGU-----------CCUGcGGCGCAAACUuuuc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 115998 | 0.66 | 0.98378 |
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Target: 5'- uGUUaGCGGG-CGCCGCcggguuuguGUUUGAAGAu -3' miRNA: 3'- -CAAcCGUCCuGCGGCG---------CAAACUUUUc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 13288 | 0.68 | 0.96155 |
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Target: 5'- cGUUGGCGGGGUGCgGCGguaGAAu-- -3' miRNA: 3'- -CAACCGUCCUGCGgCGCaaaCUUuuc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 55990 | 0.68 | 0.96155 |
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Target: 5'- --cGGaCAGuGGCGCCGCGUUggcgucGAuuAGa -3' miRNA: 3'- caaCC-GUC-CUGCGGCGCAAa-----CUuuUC- -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 88404 | 0.69 | 0.944822 |
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Target: 5'- --aGcGguGGACGCCGaGUUUGAcGAGc -3' miRNA: 3'- caaC-CguCCUGCGGCgCAAACUuUUC- -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 72743 | 0.7 | 0.911879 |
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Target: 5'- -cUGGC-GGACGaauCUGCGUUUGGAAGc -3' miRNA: 3'- caACCGuCCUGC---GGCGCAAACUUUUc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 14207 | 0.71 | 0.877207 |
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Target: 5'- -cUGGCGGGACGCCaGCacUUUGAc--- -3' miRNA: 3'- caACCGUCCUGCGG-CGc-AAACUuuuc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 32914 | 0.72 | 0.851786 |
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Target: 5'- cUUGGCAGGcgcucccauuuuCGcCCGCGUUUGggGGc -3' miRNA: 3'- cAACCGUCCu-----------GC-GGCGCAAACuuUUc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 33717 | 0.73 | 0.780892 |
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Target: 5'- -aUGGCGGGGcCGUCGCGgcUGAGu-- -3' miRNA: 3'- caACCGUCCU-GCGGCGCaaACUUuuc -5' |
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| 21205 | 5' | -50.6 | NC_004778.3 | + | 97194 | 1.09 | 0.007873 |
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Target: 5'- gGUUGGCAGGACGCCGCGUUUGAAAAGu -3' miRNA: 3'- -CAACCGUCCUGCGGCGCAAACUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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