Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
21206 | 3' | -56.9 | NC_004778.3 | + | 82378 | 0.66 | 0.877546 |
Target: 5'- uGUAguCAUGUCGAUgggaGUGGCCgGCAUu -3' miRNA: 3'- gCAUguGUGCAGCUG----CGCCGG-CGUGu -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 105198 | 0.66 | 0.877546 |
Target: 5'- uGUGgACaaACG-CGGCGCcGUCGCACAa -3' miRNA: 3'- gCAUgUG--UGCaGCUGCGcCGGCGUGU- -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 39209 | 0.66 | 0.877546 |
Target: 5'- uGUGCACguGCGUCaGCGCGGa-GCAgGg -3' miRNA: 3'- gCAUGUG--UGCAGcUGCGCCggCGUgU- -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 40815 | 0.66 | 0.877546 |
Target: 5'- aCGUcaaagACGCGCa--GACGCuGCUGCACAu -3' miRNA: 3'- -GCA-----UGUGUGcagCUGCGcCGGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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