Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21206 | 3' | -56.9 | NC_004778.3 | + | 120998 | 0.7 | 0.675057 |
Target: 5'- gGUAUugcuccaacgACAUGUCGGCgucaauuugcucuauGCGGCCGCAUu -3' miRNA: 3'- gCAUG----------UGUGCAGCUG---------------CGCCGGCGUGu -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 121425 | 0.72 | 0.517229 |
Target: 5'- --gGCACGCGUCGGcCGUGucgccgccgucggcGCCGCGCAc -3' miRNA: 3'- gcaUGUGUGCAGCU-GCGC--------------CGGCGUGU- -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 122077 | 0.75 | 0.368009 |
Target: 5'- gGUACGCG-GUCGAC-CGGCaCGCGCAu -3' miRNA: 3'- gCAUGUGUgCAGCUGcGCCG-GCGUGU- -5' |
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21206 | 3' | -56.9 | NC_004778.3 | + | 122617 | 0.67 | 0.831186 |
Target: 5'- aGgggGCGCcCGUCGugGUGuuuCCGCGCGa -3' miRNA: 3'- gCa--UGUGuGCAGCugCGCc--GGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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