Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21206 | 5' | -54.4 | NC_004778.3 | + | 42938 | 0.68 | 0.853294 |
Target: 5'- uGGUGcCGcacaggcccaUCCGCCCGACcaagauGCAGUGcUGg -3' miRNA: 3'- -CCACaGC----------AGGCGGGUUG------UGUCAC-ACa -5' |
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21206 | 5' | -54.4 | NC_004778.3 | + | 4535 | 0.69 | 0.836137 |
Target: 5'- uGGUGuUCGUUaaaucuaucacggCGCCCAGCGCGcUGUGc -3' miRNA: 3'- -CCAC-AGCAG-------------GCGGGUUGUGUcACACa -5' |
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21206 | 5' | -54.4 | NC_004778.3 | + | 29237 | 0.72 | 0.674316 |
Target: 5'- aGGcgGUCucaUCGCCCAGCAUGGUGUGa -3' miRNA: 3'- -CCa-CAGca-GGCGGGUUGUGUCACACa -5' |
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21206 | 5' | -54.4 | NC_004778.3 | + | 112070 | 0.73 | 0.591312 |
Target: 5'- --cGUCGUCuaCGCCCAugAUGGUGUGc -3' miRNA: 3'- ccaCAGCAG--GCGGGUugUGUCACACa -5' |
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21206 | 5' | -54.4 | NC_004778.3 | + | 96506 | 1.09 | 0.003411 |
Target: 5'- cGGUGUCGUCCGCCCAACACAGUGUGUu -3' miRNA: 3'- -CCACAGCAGGCGGGUUGUGUCACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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