Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21207 | 3' | -59.1 | NC_004778.3 | + | 27798 | 0.66 | 0.736384 |
Target: 5'- gCGCUGCACCGca-UGCaCGGGCcgCUUg -3' miRNA: 3'- -GCGACGUGGCaacGCG-GUCCGa-GAGa -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 61346 | 0.66 | 0.736384 |
Target: 5'- uGCUGCGUCGcugGCGCuCGGGCUgUUCg -3' miRNA: 3'- gCGACGUGGCaa-CGCG-GUCCGA-GAGa -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 95121 | 0.66 | 0.715674 |
Target: 5'- aCGCccUGCACCaGUUccagcacGCGCCGGcacuugcuGCUCUCg -3' miRNA: 3'- -GCG--ACGUGG-CAA-------CGCGGUC--------CGAGAGa -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 38741 | 0.67 | 0.666272 |
Target: 5'- gGUUGgaGCCGccGCGCauccacugCAGGCUCUCUg -3' miRNA: 3'- gCGACg-UGGCaaCGCG--------GUCCGAGAGA- -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 98864 | 0.68 | 0.574481 |
Target: 5'- uCGUUGaagaACUGUUGCGCggcacgCGGGCUUUCg -3' miRNA: 3'- -GCGACg---UGGCAACGCG------GUCCGAGAGa -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 107899 | 0.69 | 0.514775 |
Target: 5'- gGCUGCGCCGUUGCGgaaaCGGuGCUg--- -3' miRNA: 3'- gCGACGUGGCAACGCg---GUC-CGAgaga -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 25802 | 0.73 | 0.339431 |
Target: 5'- aCGCUGCcCCGccGCGCUAGuGCUgUCUg -3' miRNA: 3'- -GCGACGuGGCaaCGCGGUC-CGAgAGA- -5' |
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21207 | 3' | -59.1 | NC_004778.3 | + | 96373 | 1.07 | 0.001506 |
Target: 5'- cCGCUGCACCGUUGCGCCAGGCUCUCUu -3' miRNA: 3'- -GCGACGUGGCAACGCGGUCCGAGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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