Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21207 | 5' | -59.1 | NC_004778.3 | + | 25098 | 0.66 | 0.683716 |
Target: 5'- uGGGGUGCGCgGGUG-GCGGaGAGGGc -3' miRNA: 3'- -UUUCACGCGgUCGUgCGCCgUUCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 70276 | 0.66 | 0.673442 |
Target: 5'- --cGUGCG-CGGC-CGCGGCAuauuuaggcGGGGc -3' miRNA: 3'- uuuCACGCgGUCGuGCGCCGU---------UCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 160 | 0.67 | 0.652799 |
Target: 5'- ----aGCGCCAGCAUuuguuCGGCGuuGGGUa -3' miRNA: 3'- uuucaCGCGGUCGUGc----GCCGUu-CCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 29328 | 0.67 | 0.632089 |
Target: 5'- gAAGGUG-GCgCGGCGCGCG-CuGGGGUg -3' miRNA: 3'- -UUUCACgCG-GUCGUGCGCcGuUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 71165 | 0.67 | 0.621729 |
Target: 5'- cGGGG-GCGUUGGCGaggaaGuCGGCAAGGGUg -3' miRNA: 3'- -UUUCaCGCGGUCGUg----C-GCCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 48711 | 0.68 | 0.579423 |
Target: 5'- --cGUGCGUguuguagUAGC-CGCGGCGAcGGGUu -3' miRNA: 3'- uuuCACGCG-------GUCGuGCGCCGUU-CCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 52071 | 0.68 | 0.568163 |
Target: 5'- --cGUGCGCauugcacCAGCGCGCGGCGcgccacaAGGa- -3' miRNA: 3'- uuuCACGCG-------GUCGUGCGCCGU-------UCCca -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 1681 | 0.68 | 0.549862 |
Target: 5'- uGGGUGCgGCCAGCAgCGCGaGCAGGc-- -3' miRNA: 3'- uUUCACG-CGGUCGU-GCGC-CGUUCcca -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 112088 | 0.68 | 0.539772 |
Target: 5'- -uGGUGUGCUccucGGUGCGCaGGUAGGGGc -3' miRNA: 3'- uuUCACGCGG----UCGUGCG-CCGUUCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 81772 | 0.68 | 0.539772 |
Target: 5'- ---cUGCGCCGGUagguGCGCGGCcugccGGGGc -3' miRNA: 3'- uuucACGCGGUCG----UGCGCCGu----UCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 71249 | 0.69 | 0.509907 |
Target: 5'- cGAGG-GUGUCGGCgaagGCGuUGGCGAGGGUg -3' miRNA: 3'- -UUUCaCGCGGUCG----UGC-GCCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 39215 | 0.69 | 0.500104 |
Target: 5'- --cGUGCGUCAGCGCG-GaGCAGGGcGa -3' miRNA: 3'- uuuCACGCGGUCGUGCgC-CGUUCC-Ca -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 71310 | 0.69 | 0.500104 |
Target: 5'- gAGGGUGUcgGCgGGgGCGUaGGCGAGGGUg -3' miRNA: 3'- -UUUCACG--CGgUCgUGCG-CCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 71382 | 0.69 | 0.500104 |
Target: 5'- gAGGGUGUcgGCgGGgGCGUaGGCGAGGGUg -3' miRNA: 3'- -UUUCACG--CGgUCgUGCG-CCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 71430 | 0.69 | 0.500104 |
Target: 5'- gAGGGUGUcgGCgGGgGCGUaGGCGAGGGUg -3' miRNA: 3'- -UUUCACG--CGgUCgUGCG-CCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 66953 | 0.7 | 0.471218 |
Target: 5'- aAGAGcUGCGCCAGaacaGCGUGGuCGAGGcGg -3' miRNA: 3'- -UUUC-ACGCGGUCg---UGCGCC-GUUCC-Ca -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 46695 | 0.7 | 0.461778 |
Target: 5'- ----gGCGUCGGaauuCGCGGCGGGGGg -3' miRNA: 3'- uuucaCGCGGUCgu--GCGCCGUUCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 10836 | 0.7 | 0.452438 |
Target: 5'- ----aGCGCCuggGGCGCGuuGCGAGGGUu -3' miRNA: 3'- uuucaCGCGG---UCGUGCgcCGUUCCCA- -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 80478 | 0.71 | 0.37339 |
Target: 5'- --cGUGCGCUGGCAgCGCaucgaccccgugGGCGAGGGc -3' miRNA: 3'- uuuCACGCGGUCGU-GCG------------CCGUUCCCa -5' |
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21207 | 5' | -59.1 | NC_004778.3 | + | 129132 | 0.72 | 0.341394 |
Target: 5'- ---uUGCGCCAGCACGCGcuCAGGuGGg -3' miRNA: 3'- uuucACGCGGUCGUGCGCc-GUUC-CCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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