Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21209 | 5' | -53.8 | NC_004778.3 | + | 71340 | 0.67 | 0.912305 |
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Target: 5'- gGGGCUCGAg---GCGCgGCGgcgGCaagUCCg -3' miRNA: 3'- -CCCGAGUUuauaCGCG-CGCa--CG---AGGa -5' |
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| 21209 | 5' | -53.8 | NC_004778.3 | + | 52119 | 0.67 | 0.90605 |
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Target: 5'- aGGaCUCGccgccGAUAgcggaaGCGCGCGUGC-CCUu -3' miRNA: 3'- cCC-GAGU-----UUAUa-----CGCGCGCACGaGGA- -5' |
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| 21209 | 5' | -53.8 | NC_004778.3 | + | 39025 | 0.68 | 0.863401 |
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Target: 5'- cGGGCgacaagCAAAUuugcGCGUGCGUGCcaucuucagccaUCCa -3' miRNA: 3'- -CCCGa-----GUUUAua--CGCGCGCACG------------AGGa -5' |
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| 21209 | 5' | -53.8 | NC_004778.3 | + | 47388 | 0.68 | 0.86262 |
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Target: 5'- uGGGCgCGAcGUGUcgcacgcGCGCGCGUGgaCCa -3' miRNA: 3'- -CCCGaGUU-UAUA-------CGCGCGCACgaGGa -5' |
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| 21209 | 5' | -53.8 | NC_004778.3 | + | 57503 | 0.68 | 0.84735 |
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Target: 5'- -aGC-CGAcaugGCGCGCGUGUUCCg -3' miRNA: 3'- ccCGaGUUuauaCGCGCGCACGAGGa -5' |
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| 21209 | 5' | -53.8 | NC_004778.3 | + | 95170 | 1.1 | 0.003108 |
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Target: 5'- cGGGCUCAAAUAUGCGCGCGUGCUCCUu -3' miRNA: 3'- -CCCGAGUUUAUACGCGCGCACGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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