Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21211 | 3' | -54.9 | NC_004778.3 | + | 6847 | 0.66 | 0.923592 |
Target: 5'- cCUGUgUG-GACGCa-GGCgGCGCGUUg -3' miRNA: 3'- -GACAaACuCUGCGggUUGgCGCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 38758 | 0.66 | 0.923592 |
Target: 5'- gCUGgcUGGGcACGCCgAGaaguaCGCaGCGUCg -3' miRNA: 3'- -GACaaACUC-UGCGGgUUg----GCG-CGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 5351 | 0.66 | 0.923592 |
Target: 5'- -----gGAGGC-CgCCAACCGCGCG-Ca -3' miRNA: 3'- gacaaaCUCUGcG-GGUUGGCGCGCaG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 96227 | 0.66 | 0.917898 |
Target: 5'- uUGUgcgGAcuuGCGCCCGGCCgaGCGCGa- -3' miRNA: 3'- gACAaa-CUc--UGCGGGUUGG--CGCGCag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 64124 | 0.67 | 0.878622 |
Target: 5'- -aGUUUaGcGGCGCCCAAgCaacggucacucaGCGCGUCg -3' miRNA: 3'- gaCAAA-CuCUGCGGGUUgG------------CGCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 1677 | 0.67 | 0.878622 |
Target: 5'- -gGUUUGGGuGCGgCCAGCaGCGCGa- -3' miRNA: 3'- gaCAAACUC-UGCgGGUUGgCGCGCag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 122618 | 0.67 | 0.878622 |
Target: 5'- -----gGGGGCGCCCGucgugguguuuCCGCGCGa- -3' miRNA: 3'- gacaaaCUCUGCGGGUu----------GGCGCGCag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 90928 | 0.67 | 0.871253 |
Target: 5'- -aGUUUGcaaggcauGGACGCCgAACgCGuCGCGUUa -3' miRNA: 3'- gaCAAAC--------UCUGCGGgUUG-GC-GCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 9466 | 0.68 | 0.847837 |
Target: 5'- -cGUagGGGuuGCGCUCuGCCuGCGCGUCg -3' miRNA: 3'- gaCAaaCUC--UGCGGGuUGG-CGCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 94982 | 0.68 | 0.847837 |
Target: 5'- gCUGUUcgacGAGGCGUUCuuuCCGCGCG-Cg -3' miRNA: 3'- -GACAAa---CUCUGCGGGuu-GGCGCGCaG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 93559 | 0.68 | 0.847837 |
Target: 5'- uUGUUgGAGAUGUCCGaacuGCCGUGCa-- -3' miRNA: 3'- gACAAaCUCUGCGGGU----UGGCGCGcag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 81452 | 0.68 | 0.839616 |
Target: 5'- aUGUcUGuuGCGCgCGugCGCGCGUa -3' miRNA: 3'- gACAaACucUGCGgGUugGCGCGCAg -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 65282 | 0.68 | 0.839616 |
Target: 5'- -cGUgauGGACGCCC-ACCGCGCc-- -3' miRNA: 3'- gaCAaacUCUGCGGGuUGGCGCGcag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 2317 | 0.68 | 0.81381 |
Target: 5'- -----cGGGGCGCgCGAUCGCGUGUa -3' miRNA: 3'- gacaaaCUCUGCGgGUUGGCGCGCAg -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 82606 | 0.69 | 0.77705 |
Target: 5'- gUGUUggaUGAGGCGgCgAGCCGCcgcgcgauaGCGUCg -3' miRNA: 3'- gACAA---ACUCUGCgGgUUGGCG---------CGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 96058 | 0.69 | 0.77705 |
Target: 5'- ---gUUGGGcGCGUCCAaagACgGCGCGUCg -3' miRNA: 3'- gacaAACUC-UGCGGGU---UGgCGCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 69791 | 0.69 | 0.7675 |
Target: 5'- -----aGAGGCGgCCGugccucgcgACCGCGUGUCg -3' miRNA: 3'- gacaaaCUCUGCgGGU---------UGGCGCGCAG- -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 105556 | 0.7 | 0.748034 |
Target: 5'- --aUUUGAGACGUgcgucagcgCCGACCGCGCu-- -3' miRNA: 3'- gacAAACUCUGCG---------GGUUGGCGCGcag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 105358 | 0.7 | 0.748034 |
Target: 5'- --aUUUGAGACGUgcgucagcgCCGACCGCGCu-- -3' miRNA: 3'- gacAAACUCUGCG---------GGUUGGCGCGcag -5' |
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21211 | 3' | -54.9 | NC_004778.3 | + | 105159 | 0.7 | 0.748034 |
Target: 5'- --aUUUGAGACGUgcgucagcgCCGACCGCGCu-- -3' miRNA: 3'- gacAAACUCUGCG---------GGUUGGCGCGcag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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