Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21213 | 3' | -55.2 | NC_004778.3 | + | 105714 | 0.66 | 0.901981 |
Target: 5'- uGAUAGUggcuuacgaagCGGCGGAGaGGCGCUacaacggGGUGa -3' miRNA: 3'- gUUAUCA-----------GCCGUUUCgCCGCGG-------CCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 118803 | 0.66 | 0.896063 |
Target: 5'- aCAAUuacgaGGCccAGCGGCGCCGuGUa -3' miRNA: 3'- -GUUAucag-CCGuuUCGCCGCGGC-CAc -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 71272 | 0.66 | 0.889259 |
Target: 5'- gCGAggguGUCGGCGAGGguguCGGCGagGGUGu -3' miRNA: 3'- -GUUau--CAGCCGUUUC----GCCGCggCCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 107941 | 0.66 | 0.882219 |
Target: 5'- ---cGGcCGGCGGuGGCGGCGCCa--- -3' miRNA: 3'- guuaUCaGCCGUU-UCGCCGCGGccac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 36633 | 0.67 | 0.867451 |
Target: 5'- -----uUCGGCAAA-CGGCGCUGGc- -3' miRNA: 3'- guuaucAGCCGUUUcGCCGCGGCCac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 77124 | 0.67 | 0.859733 |
Target: 5'- -----uUUGGCGAaaaggcAGCGGCGCUGGa- -3' miRNA: 3'- guuaucAGCCGUU------UCGCCGCGGCCac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 93395 | 0.67 | 0.851801 |
Target: 5'- gGAU-GUCGuGCAcgGAGCcGaCGCCGGUGg -3' miRNA: 3'- gUUAuCAGC-CGU--UUCGcC-GCGGCCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 81922 | 0.67 | 0.851801 |
Target: 5'- ---cAGUCGGUGu--UGGCGCCGGg- -3' miRNA: 3'- guuaUCAGCCGUuucGCCGCGGCCac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 93627 | 0.68 | 0.809178 |
Target: 5'- ----uGUUGGU---GCGGCGCCGGg- -3' miRNA: 3'- guuauCAGCCGuuuCGCCGCGGCCac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 76340 | 0.68 | 0.809178 |
Target: 5'- -uGUGG-CGGCGGuugcuGCGGCGgCGGUu -3' miRNA: 3'- guUAUCaGCCGUUu----CGCCGCgGCCAc -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 34501 | 0.68 | 0.800117 |
Target: 5'- ---cGGcCGGCAAAaCGGCGUuuCGGUGg -3' miRNA: 3'- guuaUCaGCCGUUUcGCCGCG--GCCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 121999 | 0.68 | 0.789968 |
Target: 5'- gGAUGuUCGGCGgcuggugGAGUGGCGCCGa-- -3' miRNA: 3'- gUUAUcAGCCGU-------UUCGCCGCGGCcac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 114071 | 0.69 | 0.78153 |
Target: 5'- uGAgcGUCGGCgAAAGCGGUgucuugcuGCCGcGUGu -3' miRNA: 3'- gUUauCAGCCG-UUUCGCCG--------CGGC-CAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 113896 | 0.69 | 0.732786 |
Target: 5'- ----cGUCgGGCgAAGGCGGCGCCGcGg- -3' miRNA: 3'- guuauCAG-CCG-UUUCGCCGCGGC-Cac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 113994 | 0.71 | 0.660994 |
Target: 5'- -cGUGGUCGcGCgGAGGCGGCGCUGcGa- -3' miRNA: 3'- guUAUCAGC-CG-UUUCGCCGCGGC-Cac -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 12426 | 0.72 | 0.577619 |
Target: 5'- -cGUGGUCGuGCcgguGGCGGgGUCGGUGu -3' miRNA: 3'- guUAUCAGC-CGuu--UCGCCgCGGCCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 61002 | 0.73 | 0.546811 |
Target: 5'- ---cGG-CGGCGGcGGCGGCGgCGGUGg -3' miRNA: 3'- guuaUCaGCCGUU-UCGCCGCgGCCAC- -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 118129 | 0.75 | 0.439573 |
Target: 5'- -----aUCGGC--GGCGGCGCCGGUu -3' miRNA: 3'- guuaucAGCCGuuUCGCCGCGGCCAc -5' |
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21213 | 3' | -55.2 | NC_004778.3 | + | 90008 | 1.07 | 0.003579 |
Target: 5'- gCAAUAGUCGGCAAAGCGGCGCCGGUGc -3' miRNA: 3'- -GUUAUCAGCCGUUUCGCCGCGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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