Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21214 | 3' | -51.6 | NC_004778.3 | + | 98435 | 0.66 | 0.974834 |
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Target: 5'- gUUGUUCugaaacUUGGGCAGCCCGg--- -3' miRNA: 3'- aAACAGGuuc---AACUCGUCGGGCaaca -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 117415 | 0.66 | 0.971978 |
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Target: 5'- -gUGUCCGAcaaGAGCGGCuuGuUUGUg -3' miRNA: 3'- aaACAGGUUcaaCUCGUCGggC-AACA- -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 34215 | 0.66 | 0.971978 |
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Target: 5'- --aGUCCGcggcgauuuGGUUGAacacgggcaGCAGCCCGUcGg -3' miRNA: 3'- aaaCAGGU---------UCAACU---------CGUCGGGCAaCa -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 96257 | 0.66 | 0.965576 |
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Target: 5'- --aGUCCAcaaAGUacGGCGGCCCGUc-- -3' miRNA: 3'- aaaCAGGU---UCAacUCGUCGGGCAaca -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 98156 | 0.69 | 0.884664 |
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Target: 5'- -aUGUCCAAGUcgGAGCAGUCgCGc--- -3' miRNA: 3'- aaACAGGUUCAa-CUCGUCGG-GCaaca -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 21253 | 0.69 | 0.883924 |
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Target: 5'- --aGUCCGcgcggcaGGUUGAGCagauGGCCCGUg-- -3' miRNA: 3'- aaaCAGGU-------UCAACUCG----UCGGGCAaca -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 40167 | 0.75 | 0.556137 |
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Target: 5'- --aGUCCAcGUUGGGCAGCCgcgCGUUGc -3' miRNA: 3'- aaaCAGGUuCAACUCGUCGG---GCAACa -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 72815 | 0.79 | 0.370305 |
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Target: 5'- -gUGUUCGGGUUGGGCAGCCgCGUcGUg -3' miRNA: 3'- aaACAGGUUCAACUCGUCGG-GCAaCA- -5' |
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| 21214 | 3' | -51.6 | NC_004778.3 | + | 86913 | 1.05 | 0.009207 |
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Target: 5'- uUUUGUCCAAGUUGAGCAGCCCGUUGUc -3' miRNA: 3'- -AAACAGGUUCAACUCGUCGGGCAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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