Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21217 | 3' | -55.1 | NC_004778.3 | + | 74833 | 0.66 | 0.932059 |
Target: 5'- uGCGCCCguagucGGUGGCGcCGAUaGACUUc- -3' miRNA: 3'- -CGCGGG------UCAUCGCuGCUGcUUGAGca -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 68068 | 0.66 | 0.921333 |
Target: 5'- gGCGCUC---AGCGACGACGcGC-CGg -3' miRNA: 3'- -CGCGGGucaUCGCUGCUGCuUGaGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 128518 | 0.66 | 0.916191 |
Target: 5'- aGCGCCCAGUua-GACGAaauugcacgcgaaaaGGACaUCGUc -3' miRNA: 3'- -CGCGGGUCAucgCUGCUg--------------CUUG-AGCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 92592 | 0.66 | 0.915607 |
Target: 5'- cGCGCCCAuugacGUuauugAGCGA-GuCGGGCUCGa -3' miRNA: 3'- -CGCGGGU-----CA-----UCGCUgCuGCUUGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 103377 | 0.66 | 0.90964 |
Target: 5'- uCGCCUuuGGUGGCGcUGGCGccCUCGUc -3' miRNA: 3'- cGCGGG--UCAUCGCuGCUGCuuGAGCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 48713 | 0.66 | 0.903433 |
Target: 5'- uGCGUgUuguAGUAgccGCGGCGACGggUUCGc -3' miRNA: 3'- -CGCGgG---UCAU---CGCUGCUGCuuGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 52095 | 0.67 | 0.896989 |
Target: 5'- cGCGCCacaAGgAG-GACGACGAggaggACUCGc -3' miRNA: 3'- -CGCGGg--UCaUCgCUGCUGCU-----UGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 90695 | 0.67 | 0.876268 |
Target: 5'- cGUGCCCAGccAGCGGCcgguACGAA-UCGUa -3' miRNA: 3'- -CGCGGGUCa-UCGCUGc---UGCUUgAGCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 64161 | 0.67 | 0.876268 |
Target: 5'- cGCGCCC-GUGGC--CGcCGAGCUUGa -3' miRNA: 3'- -CGCGGGuCAUCGcuGCuGCUUGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 72426 | 0.67 | 0.876268 |
Target: 5'- uGCGCCacaaAGUGuaGGCGGCaAACUCGc -3' miRNA: 3'- -CGCGGg---UCAUcgCUGCUGcUUGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 10253 | 0.68 | 0.829023 |
Target: 5'- uGCGUgUGGcuUGGCGugGACGGGCcCGUg -3' miRNA: 3'- -CGCGgGUC--AUCGCugCUGCUUGaGCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 11909 | 0.68 | 0.820471 |
Target: 5'- -aGCCCauGGUGGCGAgGACGAccGCUa-- -3' miRNA: 3'- cgCGGG--UCAUCGCUgCUGCU--UGAgca -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 49158 | 0.69 | 0.784608 |
Target: 5'- aCGCCCAGUcG-GGCGAUGAGCUg-- -3' miRNA: 3'- cGCGGGUCAuCgCUGCUGCUUGAgca -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 44054 | 0.69 | 0.774332 |
Target: 5'- uCGCCCAcgcgggcGUuuGCGGCGGCGAugUCa- -3' miRNA: 3'- cGCGGGU-------CAu-CGCUGCUGCUugAGca -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 112484 | 0.7 | 0.736726 |
Target: 5'- cGCGCC----AGCGGcCGAUGAACUCGa -3' miRNA: 3'- -CGCGGgucaUCGCU-GCUGCUUGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 63410 | 0.7 | 0.736726 |
Target: 5'- uCGUCCAGUuuuugcuuuuugGGCGACGGCGAug-CGUc -3' miRNA: 3'- cGCGGGUCA------------UCGCUGCUGCUugaGCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 104191 | 0.71 | 0.696703 |
Target: 5'- uGCGCUgGGaGGCgGGCGACGAcccgGCUCGc -3' miRNA: 3'- -CGCGGgUCaUCG-CUGCUGCU----UGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 109970 | 0.73 | 0.573578 |
Target: 5'- cGCGCCCAuuacGU-GCGACGACaAGCUgGUg -3' miRNA: 3'- -CGCGGGU----CAuCGCUGCUGcUUGAgCA- -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 39403 | 0.75 | 0.474822 |
Target: 5'- gGgGCCCGGUGcUGGCGACGAAUUUGa -3' miRNA: 3'- -CgCGGGUCAUcGCUGCUGCUUGAGCa -5' |
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21217 | 3' | -55.1 | NC_004778.3 | + | 113355 | 0.75 | 0.465391 |
Target: 5'- aCGCCCgaguugaaAGUGGCGACGcGCGGGCUUGc -3' miRNA: 3'- cGCGGG--------UCAUCGCUGC-UGCUUGAGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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